The superoxide radical spin adduct of the spin trap 5,5-dimethyl-lmine-l-oxide was found to be relatively unstable in aqueous solution. The half-life of the electron spin resonance signal is approximately 80 set at pH 6 and only about 35 set at pH 8. These observations as well as the possible reaction products of 0' that may develop in the time course of an experiment, must be considered whe a ...

Double Electron-Electron Resonance (DEER) spectroscopy is a solid-state pulse Electron Paramagnetic Resonance (EPR) experiment that measures distances between unpaired electrons, most commonly between protein-bound spin labels separated by 1.5-8nm. From the experimental data, a distance distribution P(r) is extracted using Tikhonov regularization. The disadvantage of this method is that it does...

It is well established that the formation of radical species centered on various atoms is involved in the mechanism leading to the development of several diseases or to the appearance of deleterious effects of toxic molecules. The detection of free radical is possible using Electron Paramagnetic Resonance (EPR) spectroscopy and the spin trapping technique. The classical EPR spin-trapping techni...

Channelrhodopsin is a cation channel with the unique property of being activated by light. To address structural changes of the open state of the channel, two variants, which contain either 1 or 2 wild-type cysteines, were derivatised with nitroxide spin label and subjected to electron paramagnetic resonance spectroscopy. Both variants contained the C128T mutation to trap the long-lived P3(520)...

Since the spin trapping technique was developed and utilized to determine radicals in the gas phase (see reviews [1—3]), it has become attractive as a method of detecting radicals in gas-phase tobacco smoke. A first study of this sort was reported by Bluhm et al. in 1971 [4], while previous studies were forced to rely on the measurements of smoke condensate at liquid nitrogen temperature [5, 6]...

Simulating electron spin resonance spectra of nitroxide spin labels from motional models is necessary for the quantitative analysis of experimental spectra. We present a framework for modeling the spin label dynamics by using trajectories such as those from molecular dynamics (MD) simulations combined with stochastic treatment of the global protein tumbling. This is achieved in the time domain ...

We investigate the extrinsic spin Hall effect in the electron gas model due to magnetic impurities, by focusing on Ceand Yb-impurities. In the dilute limit, the skew scattering term dominates the side jump term. For Ce-impurities, the spin Hall angle αSH due to skew scattering is given by −8πδ2/7, where δ2 (≪ 1) is the phase shift for d (l = 2) partial wave. Since αSH reaches O(10 ) if δ2 > ∼ 0...