In this study, 153 clinical isolates of Shigella were screened for extended-spectrum beta-lactamase (ESBL) production by double-disk synergy test. After being confirmed by the combined disk-test and inhibitor-combined E-test strips, all positive isolates were tested for the bla gene by PCR and the enzymes by isoelectric focusing. DNA sequencing of PCR products revealed that five Shigella sonnei...

The prevalence of Escherichia coli and Klebsiella pneumoniae that produce extended-spectrum b-lactamases (ESBL) was investigated in patients of a university hospital in Split, Croatia. Patients were grouped according to age (pediatric vs. adult), antibiotic type, and hospital ward. From Jan. 2001 to Dec. 2002, the susceptibility of E. coli and K. pneumoniae isolates to antimicrobials was tested...

BACKGROUND The choice of empirical antimicrobial therapy for pneumonia in intensive care unit (ICU) is a challenge, since pneumonia is often related to multidrug-resistant pathogens, particularly extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E). To prevent the overuse of broad-spectrum antimicrobial therapy, the main objective of this study was to test the performance of dige...

Susceptibility patterns of Escherichia coli to Veterinary Critically Important Antimicrobials (VCIAs) are poorly understood in most developing countries. We determined those on n = 180 livestock fecal isolates from Chikomba district by disk diffusion method. Multiple Antibiotic Resistance (MAR) indices for the were risk analysis. Chi-square was used test how antibiotic susceptibility level asso...

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and bi...

Background & Aims: The production of extended-spectrum-β-lactamases (ESBLs) is the main mechanism of resistance to β-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to β-lactam antibiotics caused a decrease in sensitivity of the confirmatory tests for ESBL. The aim of this study was the evaluation of the β-lactamase disk test...

ISSN 2330-4456 Abstract Objectives: Neonatal sepsis with extended-spectrum betalactamase (ESBL) producing microorganisms is recognized increasingly in recent years. ESBL can be produced by various bacterial strains. This study was conducted to determine the prevalence of ESBL producing pathogens in neonatal sepsis and its impact on clinical outcome. Materials and Methods: A study was carried ou...

background: ampc and esbls as mediated-plasmid extended spectrum β-lactabases are the main factors of resistance to extended-spectrum cephalosporins in enterobacteriacea especially e. coli and will follow treatment failure, high costs of treatment in human and economic losses in the poultry industry. objectives: the purpose of this study was to screen and study the faecal e. coli isolates produ...

INTRODUCTION Delay in appropriate treatment in patients with bacteraemia can increase morbidity, mortality, and health expenditures. We compared the Rapid Direct Test (RDT) designed to detect ESBL-producing gram-negative bacteria (GNB) directly from positive blood cultures bottles, with two conventional ESBL detection tests: Screening and Confirmatory Disk Diffusion Assay (SC-DDA) and an MIC Sc...

background extended-spectrum β-lactamase (esbl) production is the major resistance mechanism to β-lactam antibiotics in enterobacteriaceae. in addition, emergence of plasmid-mediated quinolone resistance (pmqr) in esbl-producing isolates has become a global threat for treatment of these infections. objectives we investigated the association between esbl production and quinolone resistance in ur...