Microscopic discrimination between extracellular and invasive, intracellular bacteria is a valuable technique in microbiology and immunology. We describe a novel fluorescence staining protocol, called FITC-biotin-avidin (FBA) staining, which allows the differentiation between extracellular and intracellular bacteria and is independent of specific antibodies directed against the microorganisms. ...

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of myc...

The EDTA regressive staining procedure to detect RNA (Bernhard's technique) is based on the proposition that after staining ultrathin sections with uranyl the stain is preferentially removed from DNA rather than RNA by the action of the chelating agent EDTA. Whilst attempting to use the EDTA regressive staining procedure to detect the presence of RNA in the large granule complexes of chicken er...

The original technique of staining the anterior capsule of the lens with Trypan blue involves the injection of an air bubble in the anterior chamber. A drawback of this technique is the possible instability of the anterior chamber caused by the sudden exit of air when the dye is injected with the cannula through the side-port incision. Other staining techniques that use viscoelastic substances ...

faecal samples from 291 dairy calves from 10 dairy herds in kerman were examined to investigate theprevalence of cryptosporidium infection. cryptosporidium spp. oocysts were identified by using themodified ziehl-neelsen staining technique in 63 (21.65%) calves. calves were grouped according to theirage as follows:

A new technique for the staining of the corneal endothelium combines the actions of the vital stain trypan blue and the intercellular stain alizarin red S. The technique is an improvement over former staining methods, because it defines both viable and nonviable cells and thereby permits an investigator to accurately quantify endothelial cell damage.

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

background and aims. several diagnostic methods are being employed to detect benign and malignant lesions, one of which is silver nitrate staining for organizer regions. the number of nucleolar organizing regions (nors) can be used to show the degree of cell activity or metabolism in pathologic lesions. this study was designed to evaluate nors as determinants of precancerous and squamous cell c...