Preservation of original tissue dimensions is an essential prerequisite for morphometric studies. Shrinkage occurring during tissue processing for histology may severely influence the appearance of structures seen under the microscope and stereological calculations. Therefore, shrinkage has to be avoided so that estimates obtained by application of unbiased stereology are indeed unbiased. The p...

Stereological analysis is the optimal tool for quantitative assessment of brain morphological and cellular changes induced by neurotoxic lesions or treatment interventions. Stereological methods based on random sampling techniques yield unbiased estimates of particle counts within a defined volume, thereby providing a true quantitative estimate of the target cell population. Neurodegenerative d...

BACKGROUND Retinitis pigmentosa is characterized by the sequential loss of rod and cone photoreceptors. The preservation of cones would prevent blindness due to their essential role in human vision. Rod-derived Cone Viability Factor is a thioredoxin-like protein that is secreted by rods and is involved in cone survival. To validate the activity of Rod-derived Cone Viability Factors (RdCVFs) as ...

A version of the Rao-Blackwell theorem is shown to apply to most, but not all, stereological sampling designs. Estimators based on random test grids typically have larger variance than quadrat estimators; random s-dimensional samples are worse than random r-dimensional samples for s < r. Furthermore, the standard stereological ratio estimators of diierent dimensions are canonically related to e...

Ultrastructural features of cells can be fractions of a micrometer in diameter, and electron microscopy is needed to resolve them to a degree that is compatible with stereological techniques. Because the focal depth of transmission electron microscopy (TEM) images is thousands of times greater than the thickness of the sections used with TEM, virtual sectioning of sections suitable for TEM is n...

BACKGROUND Diagnosis of premalignant endometrial lesions is a major challenge to pathologists. The D-score system is more reproducible than the WHO scheme, however it is not widely available, this study attest a simple custom-made workstation for estimation of the "D-score". MATERIAL AND METHODS Fifteen endometriam with low grade hyperplasia, fifteen proliferative and eight secretory phase en...

It is well known that material chemistry and processing dictate material microstructure and microstructure influences properties and performance of materials. Consequently, quantitative characterization and statistical representation of microstructure are of considerable importance in materials science. Microstructure is essentially a collection of three-dimensional volumes (for example, grains...

Biodegradable polymers have become the materials of choice for a variety of biomedical applications. In particular, poly(DL-lactide-co-glycolide) nanoparticles have been studied as a material for drug delivery with the controlled release. In this paper we are describing a simple method for obtaining the system for targeted and controlled delivery of folic acid in the body. Folic acid was encaps...

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