The proprotein precursors of storage proteins are post-translationally processed to produce their respective mature forms within the protein storage vacuoles of maturing seeds. To investigate the processing mechanism in vivo, we isolated Arabidopsis mutants that accumulate detectable amounts of the precursors of the storage proteins, 12 S globulins and 2 S albumins, in their seeds. All six muta...

The endoplasmic reticulum (ER) is the gateway to the secretory pathway, and the proteins that are made and assembled in the ER can have a variety of cellular destinations (Vitale and Denecke, 1999). Most proteins proceed to these destinations by progression through the endomembrane system via the Golgi apparatus. This pathway of protein transport has been extensively studied in plant, mammalian...

The endoplasmic reticulum (ER) is the gateway to the secretory pathway, and the proteins that are made and assembled in the ER can have a variety of cellular destinations (Vitale and Denecke, 1999). Most proteins proceed to these destinations by progression through the endomembrane system via the Golgi apparatus. This pathway of protein transport has been extensively studied in plant, mammalian...

A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to t...

We found that membrane-less protein condensates, such as of P-granule LAF1, are able to provide an additional vehicle regulate storage and transport membrane-associated signaling proteins K-Ras4B the lipid membrane.

To study how chlorogenic acid affects changes of reactive oxygen species (ROS) and the proteins involved in ROS scavenging of nectarine during storage time, the fruits were treated with chlorogenic acid (CHA) then stored at 25°C for further studies. The CHA-treatment significantly reduced O2-· production rate, H2O2 content, and membrane permeability of nectarine fruit during storage. The key pr...

Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts in cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also to high MW proteins as well. Methods: In order to identify the most abundant zinc binding proteins in different grain tissues, we microdissected barley grains into (1) seed coats; ...

The presence of abundant storage proteins in plant embryos greatly impedes seed proteomics analysis. Vicilin (or globulin-1) is the most abundant storage protein in maize embryo. There is a need to deplete the vicilins from maize embryo extracts for enhanced proteomics analysis. We here reported a chloroform-assisted phenol extraction (CAPE) method for vicilin depletion. By CAPE, maize embryo p...