Abstract The antimicrobial effect of Cu is dependent on several factors, including the physiological state test-cultures. present study aimed to compare antibacterial newly developed Cu-coated surface, using bacterial cultures Staphylococcus epidermidis and Pseudomonas putida harvested at exponential (6h) stationary (24h) phases growth as well from a colony. Growth kinetics cell morphology were...

One of the good sources animal protein comes from meat because it contains various kinds nutrients needed by body including protein, carbohydrates, water, vitamins, fats and essential amino acids. The presence high nutritional value in can also be utilized microorganisms to grow so that reduce quality meat. Some these transmitted humans cause disease or known as zoonotic diseases. way prevent g...

We have investigated the periodic colony growth of bacterial species Bacillus subtilis. A colony grows cyclically with the interface repeating an advance (migration phase) and a rest (consolidation phase) alternately on a surface of semi-solid agar plate under appropriate environmental conditions, resulting in a concentric ring-like colony. It was found from macroscopic observations that the ch...

The effect of temperature fluctuation is an important factor in bacterial growth especially for pathogens such as the staphylococci that have to remain viable during potentially harsh and prolonged transfer conditions between hosts. The aim of this study was to investigate the response of S. aureus, S. epidermidis, and S. lugdunensis when exposed to low temperature (4°C) for prolonged periods, ...

Colony counts which approximated those in a habitat-simulating, rumen fluid-agar medium (RFM) were obtained in medium 10, a medium identical to the RFM except for the replacement of rumen fluid with 1.5 x 10(-6)m hemin, 0.2% Trypticase, 0.05% yeast extract, and a 6.6 x 10(-2)m volatile fatty acid mixture qualitatively and quantitatively similar to that in rumen fluid. Single deletion of Tryptic...

Albino mice (Rockefeller NCS strain) raised and maintained free of ordinary bacterial pathogens, as well as of intestinal Escherichia coli and of Proteus bacilli, were found to be highly resistant to the lethal effect of bacterial endotoxins. When newborn mice of this NCS colony were nursed by foster mothers from another colony raised under ordinary conditions (SS colony from which the NCS colo...

Though many diagnostic PCR methods have been described, few techniques have been developed to rapidly and efficiently screen transformed bacterial colonies for plasmid presence (1). Unfortunately, PCR screening of a large number of colonies is time consuming and requires substantial amounts of polymerase and primers. In this report, we describe a two-step large-scale bacterial colony screening ...

Proteus mirabilis colonies exhibit striking geometric regularity. Basic microbiological methods and imaging techniques were used to measure periodic macroscopic events in swarm colony morphogenesis. We distinguished three initial phases (lag phase, first swarming phase, and first consolidation phase) followed by repeating cycles of subsequent swarming plus consolidation phases. Each Proteus swa...

BACKGROUND Stethoscope diaphragms have been shown to harbor potentially pathogenic bacteria. OBJECTIVES To assess bacterial contamination on the diaphragm and under the plastic rim that secures the diaphragm of stethoscopes of physicians, nurses, medical students, and house staff in an intensive care unit and a general medical ward of a large university hospital. Also to compare the effective...

THE usual graphical method of expressing the increase of bacterial growth rate in a media by means of the bacterial number/time curve, has several disadvantages, inasmuch as that the maximum growth rate cannot be accurately determined, nor the period of optimum propagation. An improved method of presenting a bacterial growth-rate curve and eliminating the aforementioned disadvantages will now b...