A sensing method based on resonance localized surface plasmon spectroscopy was developed for low molecular weight substrate and inhibitor molecules binding to heme proteins. Cytochrome P450 proteins have Soret and Q absorption bands in the visible wavelength region. The coupling between the molecular resonance of P450 and the localized surface plasmon resonance (LSPR) of functionalized silver n...

Nanomaterials are widely used as pseudostationary and stationary phases in electrically driven separations. The advantages of using nanomaterials are numerous including tunable sizes, multiple core compositions, flexible injection schemes, and diverse surface chemistries. Nanomaterials, however, exhibit large surface energies which induce aggregation and may yield unpredictable function in sepa...

We report a new self-assembly pathway that leads to supported and hierarchically organized gold nanoparticle mesostructure arrays on solid substrates such as glass slide, thermal oxide, photopolymer film, and mica. Using the nanoparticle micelle as a building block, hierarchical gold nanoparticle mesostructure arrays were prepared by a hydrothermal nucleation and growth process through self-ass...

Tailored sensoric, electronic, photoelectrochemical, and bioelectrocatalytic functions can be designed by organized molecular or biomolecular nanoparticle hybrid configurations on surfaces. Layered receptor-cross-linked Au nanoparticle assemblies on electrodes act as specific sensors of tunable sensitivities. Layered DNA-cross-linked CdS nanoparticles on electrode supports reveal organized asse...

Oral chemotherapy is a key step towards 'chemotherapy at home', a dream of cancer patients, which will radically change the clinical practice of chemotherapy and greatly improve the quality of life of the patients. In this research, three types of nanoparticle formulation from commercial PCL and self-synthesized d-α-tocopheryl polyethylene glycol 1000 succinate (PLA-PCL-TPGS) random copolymer w...

Two β-lactoglobulin (BLG) isoforms, BLGA and BLGB, were used a test bed for the differentiation of proteins using electrostatics. In these studies, the BLGA and BLGB binding to a highly charged, cationic gold nanoparticle (GNP) modified surface was investigated by atomic force microscopy (AFM) and surface plasmon resonance (SPR) spectroscopy The binding affinity, and more importantly, the selec...

The adsorption of silica nanoparticles onto representative mineral surfaces and at the decane/water interface was studied. The effects of particle size (the mean diameters from 5 to 75 nm), concentration and surface type on the adsorption were studied in detail. Silica nanoparticles with four different surfaces [unmodified, surface modified with anionic (sulfonate), cationic (quaternary ammoniu...

cell-based biosensors have become a research hotspot in biosensors and bioelectronics fields. the main feature of cell-based biosensors is the immobilization of living cell on the surface of transducers. different types of polymers which are used as scaffolds for cell growth should be biocompatible and have reactive functional groups for further attachment of biomolecules. in this work, the cel...

Biodistribution of nanoparticles is dependent on their physicochemical properties (such as size, surface charge, and surface hydrophilicity). Clear and systematic understanding of nanoparticle properties' effects on their in vivo performance is of fundamental significance in nanoparticle design, development and optimization for medical applications, and toxicity evaluation. In the present study...