The radiosensitivities of DNA molecules from lymphocytes of human circulating blood were examined by alkaline sucrose gradient centrifugation. The single-strand breaks of DNA per rad, including the breaks formed under the present alkaline condition, were 1.2 plus or minus 0.1 breaks per 10-12 daltons DNA per rad. When the cells were transformed, the number of breaks was found to increase to 1.8...

The light-induced proton uptake of rod outer segment disc membranes has been investigated in the absence and presence of G-protein. Proton uptake was measured as the alkalisation of the suspending medium using a pH electrode and/or the indicator dye bromocresol purple. It was found that besides the known proton uptake of photolysed rhodopsin additional uptake of one proton accompanies formation...

Washed Ehrlich ascites tumor cells were suspended in either a saline or a polylysine saline solution. The cells were centrifuged, and the supernatant above the polylysinet reated or control cells or the cell pellet was studied. Inorganic phosphate, carbohydrate, free amino acids, small peptides, potassium, and adenosine-5'-monophosphate were released from the tumor cells into the supernatant af...

Human erythrocyte ghosts were loaded with 35S-labeled GSSG and with a sucrose marker, and the transport of GSSG to the suspending medium was studied. GSSG transport from ghosts occurred only when ATP was also present in the ghosts, proceeded against a concentration gradient, and was inhibited by fluoride. The rate of transport was dependent upon the intracellular concentration of GSSG. The rela...

The anaphylactic release of histamine from perfused, chopped guinea pig lung is very sensitive to changes in the NaCl concentration of the containing medium, and it is ionic strength rather than particle concentration which is critical. Consequently, in studies with inhibitors care must be taken to avoid inadvertently increasing ionic strength and thereby misinterpreting the cause of the inhibi...

Using a laser diffraction technique, we have studied factors that influence the deformability of erythrocytes. Variations in suspending medium osmolality and applied shear stress were employed to isolate the individual contributions to whole cell deformability of internal viscosity, surface area-to-volume ratio, and viscoelastic properties of the membrane. An experimental system was devised in ...

People speak not only under relaxed conditions but also during strenuous activities, and grammatical resources can be used to achieve displays of strain. This study looks at the relationship between progressivity talk bodily strain, focusing on practice temporarily suspending syntax while speaker is accomplishing a physically challenging task. Based examples from two different physical rock cli...

The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubu...