Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat ...

Nucleotides are required for a wide variety of biological processes and are constantly synthesized de novo in all cells. When cells proliferate, increased nucleotide synthesis is necessary for DNA replication and for RNA production to support protein synthesis at different stages of the cell cycle, during which these events are regulated at multiple levels. Therefore the synthesis of the precur...

Pyrimidine nucleotides are of general importance for many aspects of cell function, but their role in the regulation of biosynthetic processes is still unclear. In this study, we investigate the influence of a decreased expression of UMP synthase (UMPS), a key enzyme in the pathway of de novo pyrimidine synthesis, on biosynthetic processes in growing potato (Solanum tuberosum) tubers. Transgeni...

The red root of Salvia miltiorrhiza Bunge, a famous traditional Chinese medicine (TCM), was caused by tanshinone in epidermis cells. In order to study the biological function ncRNAs synthesis, expression patterns mRNA and were comprehensively analyzed (high content) white (low tissues derived from same plant. A total 731 differentially expressed genes (DEGs) mainly enriched primary metabolic pa...

in biotechnology has been concentrated to review alternative routes for the production of natural compounds. plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. however, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...

Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...

In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...

In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...

We examined the mechanism of ribonucleotide pool changes in methotrexate-treated Ehrlich ascites tumor cells in culture. In exponentially growing cells, methotrexate inhibited ATP and GTP formation and stimulated UTP and CTP formation by the de novo pathways. Over the following 4 h, ATP and GTP decreased and UTP and CTP increased. During this period RNA synthesis rates decreased by 30 to 40%. I...

To determine the effects of plant-based meat analogues on metabolic health and possible mechanisms, mice were fed with a real pork diet (AP), beef (AB), analogue (PP) (PB) for 68 days. Compared meat, increased food energy intake, body weight, white fat liver weight caused adipocyte hypertrophy, hepatic lipid droplet accumulation, more severe inflammatory responses in mice. Metabolomics revealed...