In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus), oocyte nuclei maintain their transcription activity during the long period o...

Rate of Circulation of the Body Fluid in Adult Tenebrio molitor Linnaeus, Anasa tristis (de Geer), and Murgantia histrionica (Hahn): Roderick Craig and N. A. Olson 648 An Instance of the Occurrence of Carcinogenic Substances in Certain Barnacles: Michael B. Shimkin, B. Kenneth Koe, and L. Zechmeister _ 650 An Analog of Histamne that Stimulates Gastric Acid Secretion without other Actions of His...

The fold of the -helical antifreeze protein from Tenebrio molitor (TmAFP) proved to be surprisingly tolerant of multiple amino acid substitutions, enabling the construction of a panel of mutants displaying grids of single amino acid types in place of the threonines on the ice-binding face. These mutants, maintaining the regularity of amino acid spacing found in the wildtype protein but with dif...

W ith the help of radioactive dilution analysis allowing the qualitative and quantitative deter­ mination of all three presently known juvenile hormones (JH-1 to 3) the following seven species of four orders were investigated in the adult stage: Coleoptera: Tenebrio molitor, Leptinotarsa decemlineata; O rthoptera: Schistocerca gregaria; B lattodea: Blatt a orientalis, Leucophaea maderae, Naupho...

Central to the conceptual basis of ecological immunity is the notion that immune effector systems are costly to produce, run, and/or maintain. Using the mealworm beetle, Tenebrio molitor, as a model we investigated two aspects of the costs of innate immunity. We conducted an experiment designed to identify the cost of an induced immune response, and the cost of constitutive investment in immuni...

The lesser peachtreeborer, Synanthedonpictipes (Grote andRobinson), is an important pest of Prunus spp.We determined the susceptibility of S. pictipes to six entomopathogenic nematode species:Heterorhabditis bacteriophoraPoinar,H. indicaPoinar, Karunakar andDavid,H.marelatusLiu and Berry, Steinernema carpocapsae (Weiser), S. feltiae (Filipjev), and S. riobrave Cabanillas, Poinar and Raulston. N...

Background: The yellow mealworm beetle, Tenebrio molitor, is a promising alternative protein source for animal and human nutrition its farming involves relatively low environmental costs. For these reasons, industrial scale production started this century. However, to optimize breed sustainable new T. molitor lines, t...

The major storage proteins in cereals, prolamins, have an abundance of the amino acids glutamine and proline. Storage pests need specific digestive enzymes to efficiently hydrolyze these storage proteins. Therefore, post-glutamine cleaving peptidases (PGP) were isolated from the midgut of the stored-product pest, Tenebrio molitor (yellow mealworm). Three distinct PGP activities were found in th...

Entomopathogenic nematodes are potent biopesticides that can be mass-produced by in vitro or in vivo methods. For in vivo production, consistently high infection rates are critical to efficiency of the process. Our objective was to optimize in vivo inoculation of Steinernema carpocapsae and Heterorhabditis bacteriophora in Galleria mellonella and Tenebrio molitor by determining effects of inocu...

The electrophysiological properties of the myogenic cardiac cells of insects have been analyzed, but the mechanisms that regulate the pacemaker activity have not been elucidated yet. In mammalian pacemaker cells, different types of membrane ion channels seem to be sequentially activated, perhaps in a cooperative fashion with the current generated by Ca(2+) extrusion mediated by the electrogenic...