BACKGROUND Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality. OBJECTIVE The aim of this investigation was to evaluate the improvement of the testicular sperm ...

Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PE...

Resumo de tese

Male factor is the sole reason or a component for infertility in 20 and 30% of cases respectively. The management of the disease may be via medical or surgical treatment. The surgical approach is classified as techniques which improves sperm production and delivery in order to achieve spontaneous pregnancy or sperm retrieval techniques prior to assisted reproductive techniques (ART). Varicocele...

Background: Male infertility refers to the inability of a male to achieve a pregnancy in a fertile female. The root of many causes of infertility is miscommunication between immune and reproductive system. Male reproductive system is very sensitive and vulnerable, infections can hinder maturation and movement of spermatozoa lead to impaired fertility.All species need an immediate reply to the m...

Background: micro-testicular sperm extraction (TESE) has been the first choice for Nonobstructive azoospermia (NOA) patients its higher retrieval rate under microscope, meanwhile, or oocyte cryopreservation are widely applied in in-vitro fertilization (IVF) treatment. But few researches have systematically explored effect of and one study. Methods: we retrospectively collected analyzed data pre...

Conventional sperm freezing methods perform best when freezing sperm samples containing at least hundreds of spermatozoa. In this severe male factor infertility case series, we examined the reproductive outcomes in 12 intracytoplasmic sperm injection cases where spermatozoa used were frozen in Cell Sleepers. Cell Sleepers are novel devices in which individual spermatozoa can be frozen in microd...

Purpose To investigate the incidence, etiology, treatment indications, and outcomes regarding infertile male patients in Japan. Methods Between April, 2014 and March, 2015, the authors contacted 47 clinical specialists in male infertility who had been certified by the Japan Society for Reproductive Medicine. The participating clinicians were sent a questionnaire regarding information on their...

BACKGROUND High-throughput studies provide a wide spectrum of genes for use as predictive markers during testicular sperm extraction (TESE) in combination with ICSI. In this work, we used the specimens from testicular biopsies of men with non-obstructive azoospermia who underwent TESE to investigate the expression of spermatogenesis-related genes MND1, SPATA22, GAPDHS and ACR. METHODS Testicu...