The classic antibiotic treatment for Mediterranean spotted fever (MSF) is based on tetracyclines or chloramphenicol, but chloramphenicol's bone marrow toxicity makes tetracyclines the treatment of choice. However, it is convenient to have alternatives available for patients who are allergic to tetracyclines, pregnant women, and children <8 years old. We conducted a randomized clinical trial to ...

Although lymphogranuloma venereum (LGV) is a serious problem in many foreign countries, it is comparatively rare in the United Kingdom. As shown by Alergant (1957), "Somewhere between a quarter and a third of all cases of LGV reported in Great Britain are first seen"-at the Seamen's Dispensary, Liverpool. A few additional cases are treated in the other regional clinics, notably at Birkenhead, w...

Tetracyclines are effective but slow-acting antimalarial drugs whose mechanism of action remains uncertain. To characterize the antimalarial mechanism of tetracyclines, we evaluated their stage-specific activities, impacts on parasite transcription, and effects on two predicted organelle targets, the apicoplast and the mitochondrion, in cultured Plasmodium falciparum. Antimalarial effects were ...

The use of medicated feed is a common practice in animal food production to improve animal health. Tetracyclines and β-Lactams are the groups that are most frequently added to this type of feed. The measurement of the concentration of the analytes in these types of samples is sometimes due to the matrix characteristic, and manufacturers are demanding fast, precise and reproducible methods. A ra...

One hundred and forty-seven Salmonella serotype Typhimurium strains isolated in three provinces in the midwest of Spain were studied. Of these, 93.6% were drug resistant. There were two predominant resistance phenotypes: 43 isolates (29.3%) were resistant to amoxicillin, tetracyclines, chloramphenicol, streptomycin and sulphamethoxazole and 27 isolates (18.4%) to amoxicillin, amoxicillin/clavul...

Here we describe a 5-step sequence to prepare the AB enone 1, the key precursor to fully synthetic tetracyclines, that begins with a diastereoselective Michael-Claisen coupling of two simple starting materials, a cyclohexenone (compound 2 or, in a refinement, a substituted variant, vide infra) and the isoxazole ester 3. This advance defines an 8-step linear sequence to 6-deoxytetracycline antib...

We have examined 221 cases of mycoplasmal pneumonia in adults during the past 17 years. During this time epidemic waves occurred every three to four years. The incidence of disease was highest in patients 20 to 30 years of age. The most common clinical features were cough, fever, sputum, and rales. The most characteristic feature was a persistent cough which lasted about three to four weeks. Ro...

An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system. Serum levels of chloramphenicol as low as 2.0 mug/ml could be reproducibly determined with thi...

The widespread dissemination of genes encoding tetracycline resistance has limited the utility of the tetracyclines for many clinical indications (Chopra, Hawkey & Hilton, 1992). There are three types of tetracycline resistance mechanisms described (Speer, Shoemaker & Salyers, 1992): 1) efflux, mediated by trans-membrane-spanning proteins, which results in reduction of the intracellular tetracy...