Bacterial production of tetrodotoxin (TTX) was first reported by Yasumoto et al. (8). Detection of bacterially produced TTX has been primarily by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). In September 1995, Applied and Environmental Microbiology published a paper indicating that both the HPLC and GC-MS methods lacked specificity for TTX (3)....
