An isocratic reversed phase stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for quantitative determination of Cetrizine hydrochloride in bulk drugs. An isocratic, reversed phase HPLC method was developed to separate the drug from the degradation products, using an Thermo Hypersil C18 (250 x 4.6)mm,5μ column and the mobile phase contai...

Staphylococcus aureus encodes two HtrA-like serine surface proteases, called HtrA1 and HtrA2. The roles of these HtrA homologs were distinguished by expression studies in a heterologous host, Lactococcus lactis, whose single extracellular protease, HtrA(Ll), was absent. HtrA(Ll) is involved in stress resistance, and processing and/or degradation of extracellular proteins. Controlled expression ...

Transglutaminase (TGase) from Streptomyces mobaraensis is a Ca(2+) independent enzyme that cross-links proteins to high molecular weight aggregates. A dispase autolysis inducing protein (DAIP) was identified as an intrinsic TGase substrate exhibiting accessible glutamine and lysine residues. DAIP modification during culture by TGase resulted in deamidation of reactive glutamines, formation of g...

Tailoring the intrinsic length-scale effects in bulk metallic glasses (BMGs) via post-heat treatment necessitates a systematic analyzing strategy. Although various achievements were made in the past years to structurally enhance the properties of different BMG alloys, the influence of short-term sub-glass transition annealing on the relaxation kinetics is still not fully covered. Here, we aim f...

Homogeneous graft copolymerization of methyl methacrylate (MMA) onto cellulose was carried out in N, N – dimethyl acetamide/LiCl (DMAc/LiCl) and dimethyl sulfoxide/ paraformaldehyde (DMSO/PF) solvent system taking ceric ammonium nitrate (CAN), benzoyl peroxide (BPO) and tin (II)-2ethyl hexanoate [Sn(Oct)2] as initiators. Different grafting parameters like graft yield (GY), grafting efficiency (...

The aim of this work is to evaluate what happens the spacecraft materials beyond End Life. A review external and effects space environment presented. This paper results from a continued study on material degradation, debris formation in geostationary orbit (GEO). In 20-year GEO dose profile that combines simultaneous UV, particles irradiation thermal cycling was applied set materials. These com...

Numerous therapeutic strategies have been developed for osteoarthritis (OA) management, including intra-articular (IA) injections. The ideal IA formulation should control cartilage degradation and restore synovial fluid viscosity. To this end, we propose to combine thermo-sensitive polymers (poloxamers) with hyaluronic acid (HA) develop suitable beta-lapachone (βLap) loaded formulations. develo...

In the present study, comprehensive stress testing of Dexketoprofen trometamol was carried out according to ICH guideline Q1A (R2). Dexketoprofen trometamol is subjected to stress conditions of hydrolysis, oxidation, photolysis and neutral decomposition. Additionally, the solid drug is subjected to 50 °C for 60 days in dry-bath, and to the combined effect of temperature and humidity, with and w...

The study examined the influence of chitosan, incorporated into whey protein and wheat starch thermo gels, on the in-vitro hydrolysis of the polysaccharide. Gels were subjected to the following external conditions containing α-amylase at constant incubation temperature of 37 °C: In the first procedure, they were immersed in phosphate buffer (0.05 M) and maintained at pH 6.9 throughout the entir...

Streptomyces sp. (strain SS12) was isolated from terrestrial soil of western U.P., India and found to exhibit antimicrobial activity against various pathogenic bacteria and fungi. Optimization of physical and chemical parameters for antibiotic production in submerged fermentation resulted in 1.75 fold increase in production level in terms of inhibition zone diameter. The bioactive compound was ...