The growth yield of Thiobacillus intermedius is greater in glucose-yeast extract or glucose-casein hydrolysate broth than in comparable media without glucose. The quantity of glucose utilized in the glucose-supplemented media is much greater than the increase in cell yield observed relative to the unsupplemented media. Addition of glucose to cell-free extracts of glucose-yeast extract or glucos...

The elemental sulfur formation by the partial oxidation of thiosulfate by both a sulfoxidizing consortium and by Thiobacillus thioparus ATCC 23645 was studied under aerobic conditions in chemostat. Steady state was attained with essentially total conversion to sulfate when the dissolved oxygen concentration was 5 mgO2 l(-1) and below a dilution rate (D) of 3.0 d(-1)for the consortium and 0.9 d(...

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. S...

effect of different amounts of sulfur fertilizer (0, 50, 100, 150, 200, 250 kg/ha) and 200 kg/ha of thiobacillus inoculant on yield, quality and storability of two edible onion cultivars red rey and white qom were investigated. the experiments were conducted at experimental field and laboratories of the department of horticulture, seed and plant improvement institute, karaj, iran in 2007-08 cro...

Compacted bentonites are one of the best sealing and backfilling clays considered for use in Deep Geological Repositories radioactive wastes. However, an in-depth understanding their behavior after placement repository is required, including if activity indigenous microorganisms affects safety conditions. Here we provide optimized phenol:chloroform based protocol that facilitates higher DNA-yie...

The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10 to 10 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidan...

sesame is one of the oldest crops in the world that is commonly cultivated in arid and semi-arid due to high oil seeds (42-52 %). this study was conducted to investigate the effects of sulfur and biological fertilizers on physiological parameters of sesame at sari agriculture sciences and natural resources university in 2014 crop year. the experiment was conducted in a split factorial design ba...

Thiobacillus thiooxidans cells became more hydrophobic but less adhesive to elemental sulfur in the presence of increasing potassium phosphate concentrations. At a fixed concentration of potassium phosphate, however, there was a peak of both cell hydrophobicity and adhesion to sulfur at around pH 5. Oxidation of sulfur by the cells was affected in a complex manner by the phosphate concentration...

Rate equations and kinetic parameters were obtained for various reactions involved in the bacterial oxidation of pyrite. The rate constants were 3.5 muM Fe per min per FeS(2) percent pulp density for the spontaneous pyrite dissolution, 10 muM Fe per min per mM Fe for the indirect leaching with Fe, 90 muM O(2) per min per mg of wet cells per ml for the Thiobacillus ferrooxidans oxidation of wash...

Partially purified preparations of ribulose-5-phosphate kinase (specific activity, 50 to 125 mumoles per min per mg of protein) were employed in a series of kinetic experiments in the presence of several concentrations of H(+), Mg(2+), adenosine triphosphate (ATP), and phosphoenolpyruvate (PEP). The pH optimum of the enzyme was found to be 7.9; at this pH and above, response of the enzyme to va...