We investigated the effect of vitamin E on membrane protein thiols under oxidative stress, which we induced by treating hepatocytes with tert-butyl hydroperoxide (TBH) for 60 mins. Those cells which we pretreated with vitamin E formed fewer blebs (22.3% compared to 60.0% in nonvitamin E-treated cells) and maintained cytosolic calcium concentration and the number of membrane protein thiols inste...

Sinusoidal efflux of hepatic reduced glutathione (GSH) is a key step in interorgan GSH/cysteine homeostasis and extracellular detoxification. Rat organic anion transporter polypeptide1 (Oatp1) is known to transport GSH but several features of sinusoidal GSH uptake, such as electrogenic property and asymmetric effects of uncharged thiols (increased efflux, decreased uptake), either cannot be acc...

Detection and quantification of different aminothiols forms (reduced and total) in biological fluids are important for the investigation of oxidative stress-related diseases and cell homeostasis study. The aim of this study was to optimize a HPLC method in order to determine both reduced and total thiol forms in red blood cells (RBC) at low temperature without washing erythrocytes. Analytical r...

Elevated oxidative stress can alter the function of proteins through the reversible oxidation of the thiol groups of key cysteine residues. This study evaluated a method to scan for reversible protein thiol oxidation in tissue by measuring reduced and oxidized protein thiols. It assessed the responsiveness of protein thiols to oxidative stress in vivo using a dystrophic (mdx) mouse model and co...

The pattern of incorporation of [14C]N-ethylmaleimide (MalNEt) into gizzard myosin indicates the presence of two classes of thiols: rapidly and slowly modified. The first class contains two thiol residues, SH-A and SH-B, located in the myosin rod and the 17-kDa light chain, respectively, while the second contains at least two thiols located in the myosin heavy chain. Changes in ATPase activitie...

Reactions of benzoylpyruvate with a mixture of aromatic aldehydes and 5-amino-1,3,4-thiadizole-2-thiol in a 1:1:1 molar ratio, afforded 5-(5-aryl-4-benzoyl-3-hydroxy-2-oxo-2,5-dihydro-1H-pyrrol-1-yl)-1,3,4-thiadizole-2-thiols (3a-c). The structure of the resulted products was confirmed by determination of the melting point and spectrophotometric techniques such as IR and 1H-NMR spectroscopy.

Cellulose sulfuric acid as a bio-polymer based solid catalyst efficiently catalyzes the selectively oxidation of sulfides to sulfoxides and thiols to disulfides using hydrogen peroxide as a green oxidant with good yields at room temperature. The developed method offers a number of advantages such as high selectivity, mild reaction conditions, simple operation, cleaner reaction profiles, low...

a general, mild and efficient protocol has been developed for the synthesis of esters and thioesters. the process has been taking place using tetra n-butylammonium iodide (tbai) as a phase-transfer catalyst and in the presence of potassium carbonate (k2co3). a wide range of esters and thioesters was prepared in high yields and suitable times by the treatment of alcohols, phenols and thiols with...

BACKGROUND We developed a sensitive, specific method for the low-molecular-mass thiols cysteine, cysteinylglycine, glutathione, and homocysteine and validated the method for measurement of glutathione in blood. METHODS The technique was based on reversed-phase chromatography (RPC) coupled on line with cold vapor generation atomic fluorescence spectrometry (CVGAFS). Thiols were derivatized bef...

Introduction: Tubular damage is common finding in acute renal failure (ARF). Various etiologies have been put forth to explain the tubular damage in ARF, one important mechanism among them is oxidative damage to renal tubules. Several biomolecules including low-molecular weight peptides and enzymes in urine have been proposed as early markers of renal failure. Current study has been undertaken ...