Lack of differentiated functions of the tissue of origin in tissue culture thought to be due to dedifferentiation was shown to be due to selective overgrowth of fibroblasts. Enrichment culture techniques, (alternate animal and culture passage), designed to give the functionally differentiated cells selective advantage over the fibroblasts resulted in a large number of functionally differentiate...

This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by ...

This study intense to identify attributes characterizing traditional farming over modern binary logistic rig-ration suggest that the silent characters were techniques of cultivation, seed used machinery used, tissue culture plants, genetically modified crops, Biofertilizers, govt. policy, and organic farming. The implication was discovered.

Since the discovery by Enders, Weller, and Robbins in 1949' that poliovirus could multiply in cultures of non-nervous tissues, cell culture techniques have been used extensively in the studies of enteroviruses' for the last decade. The accumulated literature reveals that the usefulness of these culture methods is many-fold and may include virus isolation, virus multiplication studies, and serol...

We conducted a multicenter evaluation of commercial and in-house PCR methods for the detection of enteroviruses. Three coded panels of test and control RNA samples, artificial clinical specimens, and representative enterovirus serotypes were used to assess amplification methods, RNA extraction methods, and reactivities with different enterovirus serotypes. Despite several differences between PC...

Given the importance of potato in the economy and people’s nutrition, as well as planting of disease-free seeds, it seems necessary to produce healthy seeds through tissue culture. This study was conducted during 2012-2013 at the Research Center of Agricultural University of Tajikistan aiming to evaluate the effect of different sizes of mini-tuber on yield and yield components of potato crop. A...

A new disposable micro tissue culture plate was developed and tested for use in virological procedures. Miniature mammalian cell cultures (minicultures) were grown in these plates. Each plate contained 96 circular cultures in flat wells (7 mm in diameter). Replicate titrations of a number of viruses were performed in various tissues. Excellent reproducibility was demonstrated. Mean infectivity ...