BACKGROUND Because sepsis has a high mortality rate, rapid microbiological diagnosis is required to enable efficient therapy. The effectiveness of MALDI-TOF mass spectrometry (MALDI-TOF MS) analysis in reducing turnaround times (TATs) for blood culture (BC) pathogen identification when available in a 24-h hospital setting has not been determined. METHODS On the basis of data from a total numb...

Several biologically active peptides contain a d- amino acid in a well-defined position, which is position 2 in all peptide epimers isolated to date from vertebrates and also some from invertebrates. The detection of such D- residues by standard analytical techniques is challenging. In tandem mass spectrometric (MS) analysis, although fragment masses are the same for all stereoisomers, peak int...

Nowadays, the use of sulfur dioxide (SO2) during winemaking process is a controversial societal issue. In order to reduce its use, various alternatives are emerging, in particular bioprotection by adding yeasts, with different impacts on yeast microbiota early stages. this study, quantitative-PCR and metabarcoding high-throughput sequencing (HTS) were combined MALDI-TOF-MS monitor population dy...

Metabolite profiles of white wines, including Chardonnay, Pinot gris, Riesling, Sauvignon blanc, and Viognier varieties, were determined using both gas chromatography-coupled time-of-flight mass spectrometry (GC-TOF-MS) and proton nuclear magnetic resonance spectroscopy ((1)H NMR). A total of 108 metabolites were identified by GC-TOF-MS, and 51 metabolites were identified by (1)H NMR; the major...

食品由来の酵母19菌種75株についてポテトデキストロース寒天培地, 25 °C，5日間培養した後, VITEK MSTM plusを用いてマススペクトルを取得し, SuperSpectrum (SSp) を作成した. 作成したSSpについて検証を行った結果, 同定の信頼性レベルはSchizosaccharomyces 属, Sterigmatomyces elviae, Starmerella apicola, S. etchellsii, Wickerhamomyces subpelliculosus, Zygosaccharomyces rouxii，Zygosaccharomyces bisporus では80 %以上であった. Z. bailii，Z. parabailii，Z. pseudobailii，Z. mellis, およびZ. siamensisは, それぞれのS...

OBJECTIVE To evaluate the performance of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) by using 190 Catalase-negative Gram-Positive Cocci (GPC) clinical isolates. METHODS All isolates were identified by conventional phenotypic tests following the proposed scheme by Ruoff and Christensen and MALDI-TOF MS (Bruker Daltonics, BD, Bremen, Germany). Two...