HECATE3 (HEC3) is an important transcription factor involved in regulating the carpel and transmitting tract development in Arabidopsis thaliana. The homologous gene of HEC3 in Brassica napus (designed as BnHEC3) was obtained by in silico cloning and rapid amplification of cDNA end (RACE) method. BnHEC3 obtained from cDNA is of 748 bp, containing an open reading frame (ORF) of 546bp and coding ...

We have examined the effect of the protein phosphatase inhibitors okadaic acid and microcystin on pollen-pistil interactions in Brassica. Inhibitor-treated flowers or floral buds were pollinated with untreated pollen and examined for pollen tube growth by fluorescence microscopy. Our results show that type 1 or type 2A serine/threonine phosphatases play a crucial role in the pollination respons...

Transcription factors and their associated DNA binding sites are key regulatory elements of cellular differentiation, development, and environmental response. New tools that predict transcriptional regulation of biological processes are valuable to researchers studying both model and emerging-model plant systems. SeqEnrich predicts transcription factor networks from co-expressed Arabidopsis or ...

DNA methylation is an important regulatory mechanism for gene expression that involved in the biological processes of development and differentiation in plants. To investigate the association of DNA methylation with heterosis in Brassica, a set of intraspecific hybrids in Brassica rapa and B. napus and interspecific hybrids between B. rapa and B. napus, together with parental lines, were used t...

Rapeseed (Brassica napus L.) is a crop relatively tolerant to salt. The effect of different ratios of NaCl/KCl was studied in two cultivars (Hyola308 and Kristina) of Rapeseed in 2002. Canola seedlings were grown in Hoagland’s solutions, which were salinized by different ratios of NaCl/KCl. The treatments or KCl:NaCl ratios were 0:0 (control);10:10; 20:0 (Total Added Salt or TAS=20 mol.m-3), 0:...

Sclerotinia sclerotiorum (Lib.) de Bary, the causal organism of stem rot of Brassica and over 500 host plants is distributed worldwide. Sclerotinia rot is menace to cultivation of oilseed Brassica crops in the world. Infection occurs on leaves, stems and pods at different developmental stages, causing seed yield losses of up to 80%, as well as significant reductions in oil content and quality. ...

A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP from rapeseed were perfectly matched to those of BnD22. Thus, WSCP may be identic...

this study was conducted on two canola (brassica napus) cultivars, okapi a winter type and cold tolerant and rgs003 a spring type and cold sensitive. seedlings were grown in an environmentally controlled growth room with 16 h d-1 photoperiod at 22/16 °c (day/night, control). at the 4-leaf stage, half of pots were transferred to a cold growth room for 7 d at 10/3°c (day/night, cold treatment) an...

Canola (Brassica napus L.) is one of the important sources of vegetable oils and protein-rich meals. Canola ranks third in global production of oilseed crops and fifth among economically important crop next to wheat, rice, maize, and cotton. The present work was undertaken with the aim to investigate the effect of Ethylmethyl sulphonate (EMS) on the yield contributing characters of brassica nap...

A total of 16 BnaGLN1 genes coding for cytosolic glutamine synthetase isoforms (EC 6.3.1.2.) were found in the Brassica napus genome. The total number of BnaGLN1 genes, their phylogenetic relationships, and genetic locations are in agreement with the evolutionary history of Brassica species. Two BnaGLN1.1, two BnaGLN1.2, six BnaGLN1.3, four BnaGLN1.4, and two BnaGLN1.5 genes were found and name...