A high molecular weight heteropolysaccharide, composed of glucose, glucuronic acid, N-acetylglucosamine, and mannose in an approximate molar ratio of 1:2:2:5, respectively, was isolated from phage K-2 and from the soluble fraction of phage-infected Aerobacter aerogenes lysates. Treatment of pure phage with 8 M urea at 4 degrees quantitatively solubilizes the bound polysaccharide and capsular po...

BACKGROUND Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation. Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production. Here, effect of microparticle Talc (45 μm) addition on the mycelial morphology, fermen...

Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the cell were previously discovered. In this study, we identified a cluster of po...

In earlier work, a phosphorylated derivative of muramic acid was detected upon amino acid analysis of acid hydrolysates of pneumococcal C polysaccharide. This substance has now been identified as muramic acid 6-phosphate by periodate oxidation, followed by the identification of glycolaldehyde phosphate in the oxidation mixture. Muramic acid phosphate has been found in hydrolysates of the cell w...

An experiment was conducted to study the effect of supplementing Non-starch Polysaccharide enzymes and prebiotics alone or in combination to sub-optimal energy corn soybeanbased broiler diets on performance. 150 day old broiler chicks were randomly distributed in to five experimental groups, six replicates per group and five birds per replicate. A standard diet (SD) and a basal diet (BD) (225 k...

background :  kefir contains lactic acid bacteria ( lactobacillus , lactococcus , leuconostoc , acetobacter and streptococcus ) and yeasts ( kluyveromyces , torula , candida , and saccharomyces ). kefiran is the polysaccharide produced by lactic acid bacteria in kefir.   methods :  kefiran was prepared from milk containing 0.5% fat and 10 grams kefir grains and was separated from kefir by ethan...

Five soluble polysaccharide fractions were sequentially extracted with water, EDTA, Na2CO3, 4% KOH, and 14% KOH solutions at room temperature for 4 h from cell wall material of rabbiteye blueberry (Vaccinium ashei) fruits, and their physicochemical properties were examined. The sequential treatments yielded a total 36.02% soluble polysaccharides of the dry cell wall material. HPAEC and spectros...

Protoplasts of Aureobasidium pullulans are capable of producing pullulan. Biosynthesis of the polymer pullulan required induction with kinetics similar to those of whole cells. The protoplasts also produced a heteropolysaccharide component containing mannose, glucose, and galactose. The relative proportions of the pullulan and heteropolysaccharide fractions were a function of glucose concentrat...

A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optim...

24 Caulobacter crescentus cells adhere to surfaces using an extremely strong polar 25 adhesin called the holdfast. The polysaccharide component of the holdfast is 26 comprised in part of oligomers of N-acetylglucosamine. The genes involved in the 27 export of the holdfast polysaccharide and anchoring of the holdfast to the cell were 28 previously discovered. In this study, we identify a cluster...