CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

The process of bacterial transformation results from the introduction specific genetic material into bacteria cells and subsequent gene expression. This technology has significant applications in cloning technology, allows new possibilities for breeding. use cellulose babassu mesocarp to improve was not found literature. Thus, objective this work evaluate effect biopolymers (cellulose biopolyme...

Acinetobacter baumannii is an emerging, nosocomial pathogen that is poorly characterized due to a paucity of genetic tools and methods. While whole genome sequence data from several epidemic and environmental strains have recently become available, the functional characterization of genes is significantly lagging. Efficient transformation is one of the first steps to develop molecular tools tha...

The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% – half strength, and 100% full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A....

Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the optimum cell concentration was 5 x 10(7) cells per ml. The optimum concentration of Cs+ was 1.0 M...

There are several generic sorting algorithms available that are highly optimized and are provided as part of standard programming libraries, such as the qsort implementation of quicksort available through the C standard library. In this work we present a practical algorithmic transformation that uses random sampling and ordered search, that can convert an existing implementation of any sorting ...

Reciprocal transformation reactions to streptomycin resistance have been demonstrated between pneumococcus and 2 strains of viridans streptococci, which by the usual bacteriological criteria are distantly related to pneumococcus. Desoxyribonucleic acid (DNA) extracts of a strain of streptomycin-resistant Streptococcus salivarius have been found capable of transforming pneumococcus and two strai...