During the past decade the pesticidal bacterium Bacillus thuringiensis has been the subject of intensive research. These efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism's pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. Other studies have focused on t...

Concerns have been raised regarding the preparation of Figures 4, 5, and 6 and verification of the genotypes of the three transgenic plants—P15, P19, and HcPro—which were originally described in an article by Dunoyer et al. (2004, Plant Cell) that was retracted from the published literature. These concerns were rigorously investigated, the authors have been contacted, and efforts to fully addre...

Since the maize transposon Ac can relocate to a new location within the genome, it has been used in removing selectable markers in transgenic plants. We previously developed an inducible transposon system to truncate a selectable marker in transgenic plants by locating one end of the transposon in the intron of the marker gene (glyphosate tolerant epsps gene). We have now improved upon this sys...

SaPIN2a, a plant proteinase inhibitor from nightshade (Solanum americanum), was located to the enucleate sieve elements (SEs) of phloem. The expressed SaPIN2a in transgenic lettuce showed inhibition of plant endogenous trypsin- and chymotrypsin-like activities, suggesting that SaPIN2a can regulate proteolysis in plant cells. To further investigate the physiological role of SaPIN2a, we produced ...

To understand the role of small GTPases in response to abiotic stress, we isolated a gene encoding a small GTPase, designated MfARL1, from a subtracted cDNA library in Medicago falcata, a native legume species in semi-arid grassland in northern China. The function of MfARL1 in response to salt stress was studied by expressing MfARL1 in Arabidopsis. Wild-type (WT) and transgenic plants constitut...