The sequencing, cloning, and expression of the pfp gene from Dictyoglomus thermophilum, which consists of 1,041 bp and encodes a pyrophosphate-dependent phosphofructokinase, are described. A phylogenetic analysis indicates that the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax. The recombinant and native enzymes share a high degree of similarity for mo...

background: metronidazole is drug of choice recommended by who for treatment of trichomoniasis, however, some reports claims drug resistance in trichomonas vaginalis isolates recently. the objective of this study was to determine the minimum lethal concentration (mlc) of metronidazole in resistant and sensitive strains, as well as genetic patterns of these stains by pcr method. methods : from f...

background: the aim of this study was to identify the trichomonas vaginalis strains/haplotypes based on identifying their probable variations in asymptomatic patients referred to tabriz health centers, northwestern iran. methods: sampling was taken from 50-suspected women to t. vaginalis in northwestern iran. the obtained samples were smeared and cultured. fifty dna samples were extracted, ampl...

background: the aims of this study were to examine the prevalence of trichomoniasis among women aged 18 to 48 yr in ardabil, northwestern iran and the relationship between demographic factors and the risk of infection. methods: vaginal discharge samples of 914 women aged 18 to 48 yr, referred to gynecology clinic of sabalan hospital of ardabil, iran in 2014, were collected and trichomonas vagin...