Many copies of mammalian mitochondrial DNA contain a short triple-stranded region, or displacement loop (D-loop), in the major noncoding region. In the 35 years since their discovery, no function has been assigned to mitochondrial D-loops. We purified mitochondrial nucleoprotein complexes from rat liver and identified a previously uncharacterized protein, ATAD3p. Localization studies suggested ...

A number of improvements have been added to the existing analytical model of hysteresis loop defined in parametric form. In particular, three phase shifts are included in the model, which permits to tilt the hysteresis loop smoothly by the required angle at the split point as well as to smoothly change the curvature of the loop. As a result, the error of approximation of a hysteresis loop by th...

Foul hundred eighty-six members of the Enterobacteriaceae representing nine genera were identified by conventional methods, and the results were compared with MORLUC (Biotrol Company Inc., Jamaica, N.Y.). MORLUC, an acronym for melibiose, ONPG (o-nitrophenyl-beta-galactopyranoside), rhamnose, lysine decarboxylase, urease, and citrate, are six prepackaged reagent-impregnated paper loops which ar...

In a screen for suppressors of a temperature-sensitive mutation in the yeast SNAP-25 homolog, Sec9, we have identified a gain-of-function mutation in the yeast synaptobrevin homolog, Snc2. The genetic properties of this suppression point to a specific interaction between the C-termini of Sec9 and Snc2 within the SNARE complex. Biochemical analysis of interactions between the wild-type and mutan...

Synaptic transmission requires that vesicles filled with neurotransmitter molecules be docked to the plasma membrane by the SNARE protein complex. The SNARE complex applies attractive forces to overcome the long-range repulsion between the vesicle and membrane. To understand how the balance between the attractive and repulsive forces defines the equilibrium docked state we have developed a mode...

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes form bundles of four parallel alpha-helices. The central '0' layer of interacting amino acid side chains is highly conserved and contains one arginine and three glutamines, leading to the classification of SNAREs into R, Qa, Qb, and Qc-SNAREs. Replacing one of the glutamines with arginine in the yeast exocyt...

Intracellular membrane fusion requires SNARE proteins in a trans-complex, anchored to apposed membranes. Proteoliposome studies have suggested that SNAREs drive fusion by stressing the lipid bilayer via their transmembrane domains (TMDs), and that SNARE complexes require a TMD in each docked membrane to promote fusion. Yeast vacuole fusion is believed to require three Q-SNAREs from one vacuole ...

The triple “generation, conversion and storage of energy” are the fundamental building blocks toward realizing the general aim of an energy supply on demand as unrestricted as possible. Meanwhile, however, it has become clear that this general aim leads to conflicting feedback loops on the ecological environment. Growing awareness of such deteriorating feedbacks has triggered worldwide activiti...

We consider a generalization of the representation of the so-called co-Minkowski plane (due to H. and R. Struve) to an abelian group (V;+) and a commutative subgroup G of Aut(V;+). If P = G × V satis7es suitable conditions then an invariant re8ection structure (in the sense of Karzel (Discrete Math. 208=209 (1999) 387–409)) can be introduced in P which carries the algebraic structure of K-loop ...