We investigated the hypoxemia associated with extreme leukocytosis in leukemic patients. In vitro experiments showed that the rate of decrease in the partial pressure of oxygen in the blood samples from such patients was proportional to the white cell count. In the presence of normal white cell count no drop in PO2 was observed. We conclude that a low arterial oxygen tension in the presence of ...

Molecular-scale surface roughness and charge heterogeneity have been hypothesized as factors that can affect the deposition rates of colloids during their transport in porous media. To test their relative importance, a single batch of cleaned glass beads was divided in half and chemically treated with acid or base to alter surface roughness. Analysis of the topography of 20 glass beads with an ...

Magnetic beads are convenient for extracting nucleic acid biomarkers from biological samples prior to molecular detection. These beads are available with a variety of surface functionalities designed to capture particular subsets of RNA. We hypothesized that bead surface functionality affects binding kinetics, processing simplicity, and compatibility with molecular detection strategies. In this...

UNLABELLED Lutetium oxyorthosilicate (LSO)- or lutetium-yttrium oxyorthosilicate (LYSO)-based PET scanners have intrinsic radioactivity in the scintillator crystals due to the presence of (176)Lu, which decays by beta-emission followed by one or more prompt gamma-ray emissions. This leads to intrinsic true counts that can influence the image when scanning low levels of activity. An evaluation o...

The midto long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’). Following germination on a Murashige and Skoog...

Gellan based floating beads of acetohydroxamic acid (AHA) were prepared by the ionotropic gellation method to achieve controlled and sustained drug release for treatment of Helicobacter pylori infection. The prepared beads were evaluated for diameter, surface morphology and encapsulation efficiency. Formulation parameters like concentrations of gellan, chitosan, calcium carbonate and the drug i...

Biocatalytic syntheses often require unfavorable conditions, which can adversely affect enzyme stability. Consequently, improving the stability of biocatalysts is needed, and this achieved by immobilization. In study, we aimed to compare soluble immobilized transaminases from different species. A cysteine in a consensus sequence was converted single aldehyde formylglycine-generating for directe...

The issue of antibiotic resistance among bacterial pathogens is often misunderstood. Here, we present an activity that debunks misconceptions regarding microorganisms, such as pathogens, and addresses how antibiotics work. We propose this be incorporated into middle or high school classrooms addressing both real-world elements from the Next Generation Science Standards. Students are encouraged ...

BACKGROUND Accurate detection and monitoring of disease-related biomarkers is important in understanding pathophysiology. We devised a rapid immunoreaction system that uses submicrometer polymer-coated fluorescent ferrite (FF) beads containing both ferrites (magnetic iron oxide) and fluorescent europium complexes. METHODS FF beads were prepared by encapsulation of hydrophobic europium complex...

The Faecal Egg Count Reduction Test (FECRT) is the most widely used method of assessing the efficacy of anthelmintics, and is the only in vivo technique currently approved for use with horses. Equine Faecal Egg Count (FEC) data are frequently characterised by a low mean, high variability, small sample size and frequent zero count observations. Accurate analysis of the data therefore depends on ...