Tau, a microtubule-associated protein which copurifies with tubulin through successive cycles of polymerization and depolymerization, has been isolated from tubulin by phosphocellulose chromatography and purified to near homogeneity. The purified protein is seen to migrate during electrophoresis on acrylamide gels as four closely spaced bands of apparent molecular weights between 55,000 and 62,...

305 The Rockefeller University Press $30.00 J. Cell Biol. Vol. 215 No. 3 305–307 https://doi.org/10.1083/jcb.201610066 Microtubules are cytoskeletal structures that serve as tracks for motor-based intracellular transport and underlie the organization of biological apparatuses, including the mitotic spindle, cilia, and the phragmoplast. In vivo, microtubules are highly dynamic and interconvert b...

A series of novel N-benzylbenzamide derivatives were designed and synthesized as tubulin polymerization inhibitors. Among fifty-one target compounds, compound 20b exhibited significant antiproliferative activities with IC50 values ranging from 12 to 27 nM against several cancer cell lines, possessed good plasma stability satisfactory physicochemical properties. Mechanism studies demonstrated th...

We present the design, synthesis and biological activity of novel N-substituted benzimidazole based acrylonitriles as potential tubulin polymerization inhibitors. Their was achieved using classical linear organic microwave assisted techniques, starting from aromatic aldehydes N-substituted-2-cyanomethylbenzimidazoles. All newly prepared compounds were tested for their antiproliferative in vitro...

Abstract Background and Aims Minimal change disease (MCD) is a podocytopathy more commonly seen in children, but it also accounts for 10%–25% of adult NS. Patients with MCD manifest abundant albuminuria, which related to the damage glomerular filtration barrier. Podocytes are major components barrier play crucial role maintaining integrity Alterations podocyte actin critically affect function. ...

Microtubule-associated proteins (MAPs), guanine nucleotides, and reaction temperature have substantial effects on the interaction of taxol with purified tubulin in 0.1 M glutamate. Without MAPs, taxol induced tubulin to polymerize only if GTP was present and the reaction mixture warmed. GTP was hydrolyzed in tandem with polymerization in stoichiometric amounts, while GDP inhibited both polymeri...

Formation of microtubules is a dynamic process that involves polymerization and depolymerization of αβ-tubulin heterodimers. Drugs that enhance or inhibit tubulin polymerization can destroy this dynamic process, arresting cells in the G(2)/M phase of the cell cycle. Although drugs that target tubulin generally demonstrate cytotoxic potency in the subnanomolar range, resistance due to drug efflu...

Extensive research over the last decade has resulted in a number of highly potent tubulin polymerization inhibitors acting either as microtubule stabilizing agents (MSAs) or microtubule destabilizing agents (MDAs). These inhibitors have potent cytotoxicity against a broad spectrum of human tumor cell lines. In addition to cytotoxicity, a number of these tubulin inhibitors have exhibited abiliti...