The anatomical database that describes the neural networks of the brain will eventually contain sufficient detail to identify all neurons and the individual synapses between them. One approach to obtaining this level of detail is to reconstruct brain volumes from serial electron microscopic sections. Current techniques allow the creation of relatively small ultrastructural volumes, but these co...

The ultrastructural and biochemical changes in the brain tissue of 20 mice were studied. The mice, separated into acute and chronic groups, were injected with phorbol myristate acetate (PMA) to trigger the release of free radicals. Malondialdehyde measurement and electron microscopic examination were applied for the evaluation of the effects of the free radicals. The level of lipid peroxide in ...

Quantitation of unmyelinated fibers (UF) in peripheral nerves has classically relied upon ultrastructural morphometry. Because this method is time-consuming, it is not typically performed in routine analysis of nerve biopsies. We applied the Bodian-Luxol technique to detect unmyelinated axons by light microscopy on semithin sections from resin-embedded nerve tissue. Estimates were compared to u...

The structural differences between  dormant and nondormant seeds of wild oats (Avena ludoviciana Durieu) were studied by light and transmission electron microscopy. Radial elongation of  scutellum epithelial cells were observed in nondormant seeds after imbibition, compared with dormant seeds in which cells were completely compressed. Ultrastructural differences for example, dilution of cytopla...

purpose: to report the clinico-pathological and ultrastructural features of a case of meesmann corneal dystrophy (mcd). case report: a 10-year-old boy presented with decreased vision in both eyes since early childhood. slitlamp biomicroscopy disclosed diffuse intraepithelial microcystic changes in the cornea but other layers appeared unremarkable. high pathologic myopia and amblyopia were other...

Tabular and ultrastructural studies were made of 1,000 cells, each from the thoracic duct lymph of six adult, clinically normal cats. Cells of the thoracic duct lymph were morphologically classified by visual inspection at the ultrastructural level and found to consist of 84.9% lymphocytes, 5.7% lymphoid blast cells, 6.7% immature and mature plasmacyte-like cells, 0.2% monocytes, 0.4% mitotic c...

Severe myalgia is an uncommon feature of familial Mediterranean fever (FMF). A patient is presented in whom acute myalgia and high fever were the sole clinical findings during an FMF attack. The ultrastructural picture of the muscle tissue during the acute stage was characterised by a large deposition of collagen fibrils. The myalgia subsided during colchicine treatment. The clinical and ultras...

the identification of mouse basophils as interleukin-4 (IL-4)– producing, high-affinity FceR-positive, histamine-containing cells sorted from mouse bone marrow and spleen,3 previously referred to as non-B, non-T cells.10 The ultrastructural characteristics of these basophils are described and contrasted with those of FceR-positive mast cells and with FceR-negative neutrophils and eosinophils in...

An argon ion laser microbeam (488 and 514 nm) was used to selectively irradiate one of the two centriolar regions of rat kangaroo Potorous tridactylis (PtK2) prophase cells in vitro. The cells were sensitized to the laser radiation by treatment with acridine orange (0.1-0.2 mug/ml). Ultrastructural examination of the irradiated centriolar regions demonstrated that the primary site of damage was...

Secondary hyphae of Lyophyllum ulmarium were shown to tolerate slow freezing, which allowed extracellular freezing, to -196 degrees C. A freeze-fracture study showed that under this non-lethal freezing condition, the plasma membrane of the secondary hyphae did not show any ultrastructural changes as compared with the control, except gross cellular shrinkage. Tertiary hyphae of Lyophyllum ulmari...