نتایج جستجو برای: unfolding

تعداد نتایج: 11072  

Journal: :The Biochemical journal 1998
L A Wallace G L Blatch H W Dirr

A topologically conserved residue in alpha-helix 6 of domain II of human glutathione transferase (hGST) A1-1 was mutated to investigate its contribution to protein stability and the unfolding pathway. The replacement of Leu-164 with alanine (L164A) did not impact on the functional and gross structural properties of native hGST A1-1. The wild-type protein unfolds via a three-state pathway in whi...

2012
Seth Lichter Benjamin Rafferty Zachary Flohr Ashlie Martini

All-atom explicit-solvent molecular dynamics simulations are used to pull with extremely large constant force (750-3000 pN) on three small proteins. The introduction of a nondimensional timescale permits direct comparison of unfolding across all forces. A crossover force of approximately 1100 pN divides unfolding dynamics into two regimes. At higher forces, residues sequentially unfold from the...

Journal: :Biophysical Journal 2008
Giovanni Settanni Alan R. Fersht

We report high temperature molecular dynamics simulations of the unfolding of the TRPZ1 peptide using an explicit model for the solvent. The system has been simulated for a total of 6 mus with 100-ns minimal continuous stretches of trajectory. The populated states along the simulations are identified by monitoring multiple observables, probing both the structure and the flexibility of the confo...

Journal: :Biophysical journal 2002
Daniel J Müller Max Kessler Filipp Oesterhelt Clemens Möller Dieter Oesterhelt Hermann Gaub

The combination of high-resolution atomic force microscopy imaging and single-molecule force spectroscopy allows the identification, selection, and mechanical investigation of individual proteins. In a recent paper we had used this technique to unfold and extract single bacteriorhodopsins (BRs) from native purple membrane patches. We show that subsets of the unfolding spectra can be classified ...

Journal: :Journal of molecular biology 2008
Yi Cao Hongbin Li

We present the first single-molecule atomic force microscopy study on the effect of chemical denaturants on the mechanical folding/unfolding kinetics of a small protein GB1 (the B1 immunoglobulin-binding domain of protein G from Streptococcus). Upon increasing the concentration of the chemical denaturant guanidinium chloride (GdmCl), we observed a systematic decrease in the mechanical stability...

Journal: :Biophysical journal 2003
Richard Law Philippe Carl Sandy Harper Paul Dalhaimer David W Speicher Dennis E Discher

Force-driven conformational changes provide a broad basis for protein extensibility, and multidomain proteins broaden the possibilities further by allowing for a multiplicity of forcibly extended states. Red cell spectrin is prototypical in being an extensible, multidomain protein widely recognized for its contribution to erythrocyte flexibility. Atomic force microscopy has already shown that s...

Journal: :CoRR 2012
Joseph O'Rourke

We address the unsolved problem of unfolding prismatoids in a new context, viewing a “topless prismatoid” as a convex patch—a polyhedral subset of the surface of a convex polyhedron homeomorphic to a disk. We show that several natural strategies for unfolding a prismatoid can fail, but obtain a positive result for “petal unfolding” topless prismatoids. We also show that the natural extension to...

Journal: :Biophysical journal 2000
Z Bryant V S Pande D S Rokhsar

Single-molecule mechanical unfolding experiments have the potential to provide insights into the details of protein folding pathways. To investigate the relationship between force-extension unfolding curves and microscopic events, we performed molecular dynamics simulations of the mechanical unfolding of the C-terminal hairpin of protein G. We have studied the dependence of the unfolding pathwa...

Journal: :Journal of the American Chemical Society 2015
Xinyue Zhang Xiaojun Xu Zhiyu Yang Andrew J Burcke Kent S Gates Shi-Jie Chen Li-Qun Gu

Pseudoknots are a fundamental RNA tertiary structure with important roles in regulation of mRNA translation. Molecular force spectroscopic approaches such as optical tweezers can track the pseudoknot's unfolding intermediate states by pulling the RNA chain from both ends, but the kinetic unfolding pathway induced by this method may be different from that in vivo, which occurs during translation...

2008
Doina Cioranescu Patrizia Donato Rachad Zaki D. Cioranescu A. Damlamian DOINA CIORANESCU PATRIZIA DONATO RACHAD ZAKI

The periodic unfolding method was introduced in [4] by D. Cioranescu, A. Damlamian and G. Griso for the study of classical periodic homogenization. The main tools are the unfolding operator and a macro-micro decomposition of functions which allows to separate the macroscopic and microscopic scales. In this paper, we extend this method to the homogenization in domains with holes, introducing the...

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