نتایج جستجو برای: using rnease mini kit
تعداد نتایج: 3426840 فیلتر نتایج به سال:
Antisense RNA probes were synthesized by in vitro transcription incubating 1 μg linearized plasmid, 2 μL 100 mM dithiothreitol (DTT), 1.3 μL NTP mixture (15.4 mM each ATP, CTP, GTP, and 10 mM UTP), 0.7 μL 10 mM digoxigenin-UTP or fluorescein-UTP (Roche Applied Science, Indianapolis, IN, USA), and 0.5 μL RNAguardTM (Amersham Biosciences, Piscataway, NJ, USA), 2 μL 10× transcription buffer, 1 μL ...
Background: There are many genetic and non-hereditary risk factors that are known to causes venous thromboembolic (VTE) disorders, Cardiovascular diseases and types of cancer. One of these is the Prothrombin G20210A mutation. Prothrombin mutation (guanine to adenine G→A) at nucleotide position 20210, which is present in the 3′ untranslated region of the prothrombin gene. Prothrombin G20210A mut...
BACKGROUND Brucellosis is a zoonosis disease which is widespread across the world. OBJECTIVES The aim of the present study is the evaluation of culture-negative blood samples. MATERIALS AND METHODS A total of 100 patients with suspected brucellosis were included in this experimental study and given positive serological tests. Diagnosis was performed on patients with clinical symptoms of the...
background: recently, some new viruses have been identified for their association with hepatitis which torque teno mini virus being among them. the aim of this study was to determine the frequency of torque teno mini virus in healthy individuals and hepatitis b and c patients in isfahan, iran. materials and methods: one hundred serum samples of healthy individuals from isfahan blood transfusion...
AIMS Sensitive diagnosis of Acanthamoeba infections may prevent the clinical condition from becoming worse. In order to improve the diagnosis tool performances, we studied the implication of the DNA extraction procedures on the detection of Acanthamoeba by real-time PCR. METHODS Acanthamoeba cysts mixed with a tag virus were processed according to different DNA preparation procedures: heat, P...
The pfcrt gene is a biomarker to determine the resistance of Plasmodium falciparum chloroquine and amodiaquine. When was switched ACT, it very likely that there will be an increase in wild type strains (pfcrt K76) due absence exposure chloroquine. Currently has not used for malaria treatment. aims this study were identify polymorphisms phylogenetic analysis isolates from patients Pesawaran Rege...
We have established a PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with primers specific to adhesin subunit gene. The minimum amount of H. pylori DNA that could be detected was 0.4 pg. Seventy-two antral biopsy specimens were taken from patients with gastritis and/or duodenal ulcer. H. pylori was found in 58.3%, 44.4% and 54.2% of patients according to the resu...
Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) an...
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. Egg precursor cells (EPCs), isolated from the ovarian cortex, have the potential to be used to overcome female infertility. We aimed to define the origins of EPCs by analyzing their gene expression profiles and mtDNA content using a mini-pig model. We characterized FAC-sorted DDX4+-derived porcine EPCs...
cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite cryptosporidium parvum. water–borne transmission of this organism has become more prevalent in recent years. current method for detection of c. parvum oocysts in water is immunofluoresence assay (ifa). the method is time consuming, laborious and particularly not–specific. it cannot determine the infectivity of detecte...
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