Activated protein C (APC) inhibits coagulation by cleaving and inactivating procoagulant factor Va (FVa) and factor VIIIa (FVIIIa). FV, in addition to being the precursor of FVa, has anticoagulant properties; functioning in synergy with protein S as a cofactor of APC in the inhibition of the FVIIIa-factor IXa (FIXa) complex. FV:Q506 isolated from an individual homozygous for APC-resistance is l...

Multiple host genes have been shown to influence the course of erythroleukemia induced by Friend virus (FV) 1 (1). Two H-2-associated genes and a non-H-2-1inked gene are required for recovery from FV leukemia (2, 3). The non-H-2-1inked gene, Rfv-3, appears to influence the production of anti-FV antibody independent of the H-2 genotype (3). Although mice of the Rfv-3 r/~ genotype produce high le...

Selection of antibody library in vitro is almost always performed on a certain solid-phase with immobilized antigen. However, for the selection of small molecule binders, conjugation of the antigen to a carrier molecule is indispensable, which often leads to the selection of unwanted binders such as conjugate-binders or those with insufficient specificity. Here we describe a rapid and efficient...

Multispecific antibody formats provide a promising platform for the development of novel therapeutic concepts that could facilitate the generation of safer, more effective pharmaceuticals. However, the production and use of such antibody-based multispecifics is often made complicated by: 1) the instability of the antibody fragments of which they consist, 2) undesired inter-subunit associations,...

Chlorophyll fluorescence has been used to predict and monitor coral bleaching over short timescales (hours to days), but long-term changes during recovery remain largely unknown. To evaluate changes in fluorescence during long-term bleaching and recovery, Porites compressa and Montipora capitata corals were experimentally bleached in tanks at 30 degrees C for 1 month, while control fragments we...

Activated protein C (APC) inhibits coagulation by cleaving and inactivating procoagulant factor Va (FVa) and factor VIIIa (FVIIIa). FV, in addition to being the precursor of FVa, has anticoagulant properties; functioning in synergy with protein S as a cofactor of APC in the inhibition of the FVIIIafactor IXa (FIXa) complex. FV:Q506 isolated from an individual homozygous for APC-resistance is le...

The effects of artificially applied acid rain on functional activity of PS2 and oxygen evolution of bean plants (Phaseolus vulgaris L.) were investigated. The plants were subjected to single treatment with acid rain of different pH – pH 5.6 (control) and 2.4, 2.2, 2.0 and 1.8. Measurements were carried out in dynamics – at time intervals of 3–168 hours. The results showed that the treatment of ...

Fv-4 is a mouse gene that dominantly confers resistance to infection with Friend murine leukemia virus (F-MuLV) (S. Suzuki, Jpn. J. Exp. Med. 45:473-478, 1975). However, the resistance caused by Fv-4 is recessive in nude mice, which suggests that immunological effects play important roles in this resistance in vivo (K. Higo, Y. Kubo, Y. Iwatani, T. Ono, M. Maeda, H. Hiai, T. Masuda, K. Kuribaya...

Foamy virus (FV) vectors are promising tools for gene therapy, but low titer is a major challenge for large-scale clinical trials. Here, we increased FV vector titer 50-fold by constructing novel vector plasmids and using polyethylenimine-mediated transfection. FV and lentiviral (LV) vectors were used separately to transduce human CD34(+) cells at multiplicities of infection of 25, and those ce...

generation at the activated platelet surface. What remains uncertain, however, is whether residual platelet FV levels correlate with the severity of bleeding in severe FV-deficient patients. Although there has been speculation on this correlation,8 no systematic study has been done. Furthermore, mechanistic studies are needed to investigate some remaining questions. The first relates to the ori...