Objective: Methotrexate (MTX) is a commonly used chemotherapeutic agent in clinical oncology practice and treatment of autoimmune diseases. The major side effect during mucositis. Recently published studies reported that this may be due to direct intestinal epithelial injury disturbance microbiota. In study, we aimed characterize the histological changes caused by MTX investigate effects safety...

CELIAC DISEASE DEVELOPS IN GENETICALLY PREDISposed individuals as a response to ingested gluten. Gluten is the term for the storage proteins of the cereal grains wheat, rye, and barley. While virtually 100% of the population ingests these grains in one form or another and 30% to 40% of the population carry the genetic markers of the disease (HLA-DQ2 or type 8), only 2% to 3% of those with genet...

We have examined the effects of parasite infection on the mucosal architecture of mice maintained on an elemental diet (Vivonex). Techniques used were conventional histology, micro-dissection and measurement of individual villi and crypts, and measurement of crypt cell proliferation rate by a metaphase accumulation technique. In normal, non-parasitised mice the elemental diet caused no change i...

BACKGROUND Villous atrophy, mucin changes ('colonic metaplasia'), and chronic inflammation occur to varying degrees in all patients with ileal pouchanal anastomosis whereas acute inflammation (pouchitis) affects a subgroup of patients with prior ulcerative colitis. AIM To measure epithelial barrier function looking for possible functional adaptation in ileal 'pouch' mucosa. PATIENTS Patient...

AIM To use a computerized shape-from-shading technique to characterize the topography of the small intestinal mucosa. METHODS Videoclips comprised of 100-200 images each were obtained from the distal duodenum in 8 celiac and 8 control patients. Images with high texture were selected from each videoclip and projected from two to three dimensions by using grayscale pixel brightness as the Z-axi...

Intestinal permeability to probe molecules has been shown to correlate closely with the presence or absence of villous atrophy in a jejunal biopsy. The purpose of this study was to establish if there exist groups of patients with functional derangement of intestinal permeability but normal histopathology of the small bowel mucosa. In 135 patients a cellobiose/mannitol permeability test was perf...

Tufting enteropathy First described in 1994, tufting enteropathy is a clinicopathological entity in which infants present with watery diarrhea in the first month of life. The pattern of inheritance appears to be autosomal recessive. Jejunal biopsy shows partial or subtotal villous atrophy with normal or hyperplastic crypts. The lamina propria is not inflamed and intraepithelial lymphocytes are ...