The complete DNA sequence of herpes B virus (Cercopithecine herpesvirus 1) strain E2490, isolated from a rhesus macaque, was determined. The total genome length is 156,789 bp, with 74.5% G+C composition and overall genome organization characteristic of alphaherpesviruses. The first and last residues of the genome were defined by sequencing the cloned genomic termini. There were six origins of D...

HIGHLIGHTS A Feature extraction technique is proposed which extracts 120 features from Corona virus genome sequences. classification accuracy of 97.96% achieved for a dataset with 1000 samples seven strains The most repeat pattern “TTGTT” identified in COVID-19 sequence.

In this research, we demonstrated a microfluidic chip to pretreat the samples for viral genome assay. The microfluidic chip has the following three functions; (1) Virus purification and enrichment, (2) Viral RNA extraction, and (3) Capture of the targeted virus genome. Hydroxyapatite chromatography, Boom method, and PNA (Peptide Nucleic Acid) were used for the above three functions, respectivel...

background: the present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (hsv), cytomegalovirus (cmv), and epstein-barr virus (ebv) in crc and colorectal polyp by using the pcr method in iran. methods: in this analytical case-control study, we selected 15 patients with crc, 20 patients with colorectal polyp, and 35 patients without mali...

In genome analysis, it is often important to identify variants from a reference genome. However, identifying that occur with low frequency can be challenging, as computationally intensive do so accurately. LoFreq widely used program adept at variants. This paper presents design framework for an FPGA-based accelerator LoFreq. particular, this targeted virus which particularly compared human the ...

We analyzed the virus shedding of an oseltamivir-treated patient who had been infected with the pandemic swine-origin influenza A (H1N1) virus which had an oseltamivir-sensitive neuraminidase. The virus was isolated from the pharyngeal swabs of the patient using MDCK cells, and the virus genome RNA was detected in the same samples both by real-time RT-PCR and RT-PCR. The virus was isolated unti...