Freeze-thawing cat sperm in cryoprotectant results in extensive membrane damage. To determine whether cooling alone influences sperm structure and viability, we compared the effect of cooling rate on sperm from normospermic (N; > 60% normal sperm per ejaculate) and teratospermic (T; < 40% normal sperm per ejaculate) domestic cats. Electroejaculates were divided into raw or washed (Ham's F-10 + ...

Artificial insemination (AI) of pigs has grown exponentially as reproductive technology with more than a dozen of the top pig producers of the world inseminating >95% of the females in production schemes, at either nucleus or multiplication herds. Above 98% of AIs still concern liquid semen, often inseminated just within a day or maximum two from collected and extended, and most often using cer...

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing 500 μl mTCM199 maturation medium. The sperm rich portion of ejacul...

Abstract Study question Do sperm mitochondrial DNA copy number (mtDNAcn) and function correlate with fertilization rates after intracytoplasmic injection (ICSI)? Summary answer We reveal a negative correlation between mtDNAcn ICSI, while higher corresponded to an increase in capacity. What is known already Over 40% of male-factor infertility unknown origin (unexplained idiopathic nature). As cu...

Gayo area is the largest Arabica coffee production in Asia. The materials used this research were beans which are natural, semi-washed, and fully-washed processed coffee. This study aimed to determine physicochemical of green bean will various processing. moisture content three 9.78%, 9.60% 11.03% for semi-washed full-washed processing, respectively. ash ranged from 3.19% natural processing 4.3...

Incubation of hamster eggs with trypsin or chymotrypsin, but not with a series of glycosidases or lipases, prevents capacitated spermatozoa from binding to the zona pellucida so that fertilization is prevented (Hartmann & Gwatkin, 1971 ; Gwatkin, Williams & Andersen, 1973). Inactivation of the binding sites for spermatozoa on the zona pellucida by a trypsin-like protease released by discharge o...