Protein methylase I (S-adenosyl-L-methionine:protein (arginine) N-methyltransferase; EC 2.1.1.23) has been purified from wheat germ approximately 90-fold with a yield of 160%. This unusually high yield of the enzyme activity suggested the presence of an inhibitor. Subsequently, this inhibitor was purified to homogeneity and was found to be identical with adenosine; W absorption spectrum, retent...

Pectate lyase (PL) was produced by the filamentous fungus Penicillium viridicatum RFC3 in solid-state cultures of a mixture of orange bagasse and wheat bran (1 : 1 w/w), or orange bagasse, wheat bran and sugarcane bagasse (1 : 1 : 0.5 w/w), and in a submerged liquid culture with orange bagasse and wheat bran (3%) as the carbon source. PL production was highest (1,500 U mL(-1) or 300 Ug(-1) of s...

BACKGROUND Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected to investigate the enzymatic system involve...

Wheat (Triticum aestivum) storage proteins fold and assemble into complexes that are linked by intra- and intermolecular disulfide bonds, but it is not yet clear whether these processes are spontaneous or require the assistance of endoplasmic reticulum (ER)-resident enzymes and molecular chaperones. Aiming to unravel these processes, we have purified and characterized the enzyme protein disulfi...

The study aimed at isolating a potential acid protease producing fungal strain from local soil source. The fungal strains were isolated from garden soil on the basis of clearance zone on casein-glycerol agar flooded by coomassie blue stain. Aspergillus sp. showing maximum clearance on casein agar plates (pH 5) was selected for further studies. Optimization of various factors influencing maximum...

Cinnamyl alcohol dehydrogenase (CAD) catalyses the final step in the biosynthesis of monolignols. In the present study, a cDNA encoding a CAD was isolated from wheat, designated as TaCAD1. A genome-wide data mining in the wheat EST database revealed another 10 CAD-like homologues, namely TaCAD2 to TaCAD11. A phylogenetic analysis showed that TaCAD1 belonged to the bona fide CAD group involved i...

Fructans, beta2-1 and/or beta2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves, widely distributed in plants, are believed to be mobilized via fructan exohydrolases (FEHs). The purification, cloning, and functional characterization of a 6-FEH from wheat (Triticum aestivum L.) are r...

Article Type: Full Length Research Article This research highlighted the production of cellulase enzyme from isolated fungal strains and used this enzyme as potential refining agent in manufacturing security paper from cotton comber pulp. Twenty three fungal strains were isolated from soil samples collected from nearby wood based paper mill and screened for cellulolytic activity by Congo red pl...

Soft rot (type II) fungi belonging to the family Xylariaceae are known to substantially degrade hardwood by means of their poorly understood lignocellulolytic system, which comprises various hydrolases, including feruloyl esterases and laccase. In the present study, several members of the Xylariaceae were found to exhibit high feruloyl esterase activity during growth on lignocellulosic material...

Wheat (Trificum aestivum) storage proteins fold and assemble into complexes that are linked by intraand intermolecular disulfide bonds, but it is not yet clear whether these processes are spontaneous or require the assistance of endoplasmic reticulum (ER)resident enzymes and molecular chaperones. Aiming to unravel these processes, we have purified and characterized the enzyme protein disulfide ...