A lipase producing bacterium, Bacillus cereus MSU AS was isolated from the gut of a marine fish Sardinella longiceps. In total, seven agricultural waste substrates (coconut oil cake, neem oil cake, onion skin waste, wheat bran, jack fruit waste, banana skin peels and pomegranate skin peel) were selected for solid state production of lipase by B. cereus MSU AS. Among the tested substrates, onion...

UNLABELLED Carob germ proteins have been shown to have functional properties similar to wheat gluten enabling formulation and production of yeast leavened gluten-free baked goods from a true dough rather than a stiff batter. The purpose of this research was to optimize the production of wheat-free bread containing carob germ flour, corn starch, NaCl, sucrose, hydroxypropyl methylcellulose (HPMC...

Two protein phosphatases (enzymes I and II) were extensively purified from wheat embryo by a procedure involving chromatography on DEAE-cellulose, phenyl-Sepharose CL-4B, DEAE-Sephacel and Ultrogel AcA 44. Preparations of enzyme I (Mr 197,000) are heterogeneous. Preparations of enzyme II (Mr 35,000) contain only one major polypeptide (Mr 17,500), which exactly co-purifies with protein phosphata...

A soluble enzyme system which catalyzes the aerobic oxidation of reduced pyridine nucleotides in wheat germ has previously been described (1). Peroxidase was shown to be a component of this system. Later, Humphreys (2) demonstrated that Mn+ and an unidentified cofactor were required for aerobic oxidation of TPNH’ in crude preparations of malic enzyme from wheat germ. Although no attempt was mad...

An extract of fermented wheat germ (EFWG) was prepared by treatment with Aspergillus oryzae and in vitro antioxidant and cellular antioxidant capacities were measured. The induction of phase II detoxifying and antioxidant enzymes in exerting a cellular antioxidant capacity was determined. Treatment of wheat germ with A. oryzae significantly (p<0.001) increased the ferulic acid content, compared...

The mutant strain Trichoderma reesei WX-112 with high cellulase activity was isolated by a newly invented plate. The mutant’s ability to produce cellulase increased 1.95 times after the treatment with UV and N-methyl-N’-nitro-N-nitrosoguanidine (MNNG). Also, the medium composition was optimized using response surface methodology (RSM). A fractional factorial design (2) was applied to elucidate ...