Cereals play a significant role in the Ukrainian economy and agriculture. Viral diseases can cause serious reduction yields. Aim. The aim of work was to identify determine spread grain viruses main cereal-growing regions Ukraine period 2020–2021 using different methods virus detection. Methods. Enzyme-linked immunosorbent assay used commercial test systems by for wheat streak mosaic (WSMV), bar...

Seifers, D. L., and Martin, T. J. 1988. Correlation of low level wheat streak mosaic virus resistance in Triumph 64 wheat with low virus titer. Phytopathology 78:703-707. Wheat streak mosaic virus (WSMV) reduced the yield of Triumph 64 immunosorbent assay (ELISA). As incubation temperature increased from (cultivar having a low level of resistance to virus multiplication) by 18% 15 to 30 C, diff...

Monopartite members of the family Potyviridae utilize three virus-encoded proteinases to cleave the viral polyprotein into mature proteins. The amino-terminal region of the viral polyprotein is autolytically cleaved by the P1 proteinase. A domain required for P1 proteinase activity of Wheat streak mosaic virus (WSMV) was mapped using a series of templates with nested 3'-truncations or 5'-deleti...

Abstract Wheat streak mosaic virus (WSMV) is a common wheat causing economic losses to production in the Great Plains of North America. Reproducible inoculation WSMV by mechanical methods essential evaluate resistance breeding lines and relies on successful infectivity particles. We used reverse transcription-quantitative PCR (RT-qPCR) for absolute quantification viral genome copy numbers both ...