The utility of the pfxa3 probe for direct molecular diagnosis of the fragile X (FRAXA) has been established. This probe detects amplification of an unstable DNA element consisting of variable length CCG repeats. The size of the amplified fragment is correlated with phenotype and was determined using PstI digested DNA in family members. In 35 families with the fragile X, there was correspondence...

It has been suggested that delayed DNA replication underlies fragility at common human fragile sites, but specific sequences responsible for expression of these inducible fragile sites have not been identified. One approach to identify such cis-acting sequences within the large nonexonic regions of fragile sites would be to identify conserved functional elements within orthologous fragile sites...

background: fragile histidine triad protein (fhit), as a known tumor suppressor protein, has been proposed to play crucial role in inhibiting p53 degradation by mdm2. studies have confirmed fhit interaction with p53 or mdm2, although functional interacting domains of fhit with mdm2 and/or p53 are not completely defined. thus, through determining the significant structural interacting domains of...

Introduction: Fragile X syndrome (FXS) is one of the most prevalent genetic causes of developmental disability, representing the most frequent form of inherited severe cognitive deficit. The present study was undertaken to investigate FXS and its prevalence in moderate mentally retarded people in patients. Materials and methods: Nineteen people with moderate mental retardation (MR) who wer...

Innovation in materials and technologies has promoted the fabrication of thin-film electronics on substrates previously considered incompatible because their chemical or mechanical properties. Indeed, conventional processes, typically based photolithography, involve solvents acids that might harm fragile exotic substrates. In this context, transfer techniques define a route to overcome issues r...

Interaction-free measurement (IFM)-based single-photon interference is developed as a powerful platform for revealing object information in quantum metrology, where the presence of sample identified without photon “touching” an interferometer. Herein, method obtaining spectroscopic properties with interaction-free spectroscopy (IFQS) based on spectrum-correlated pairs by spontaneous parametric ...

It has recently emerged that the succinimide linkage of a maleimide thiol addition product is fragile, which is a major issue in fields where thiol functionalisation needs to be robust. Herein we deliver a strategy that generates selective cysteine thiol labelling reagents, which are stable to hydrolysis and thiol exchange.

It has been suggested that delayed DNA replication underlies fragility at common human fragile sites, but specific sequences responsible for expression of these inducible fragile sites have not been identified. One approach to identify such cis-acting sequences within the large nonexonic regions of fragile sites would be to identify conserved functional elements within orthologous fragile sites...

In contrast to synthetic materials, evolutionary developments in biology have resulted in materials with remarkable structural properties, made out of relatively weak constituents, arranged in complex hierarchical patterns. For instance, nacre from seashells is primarily made of a fragile ceramic, yet it exhibits superior levels of strength and toughness. Structural features leading to this per...