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Total RNA was extracted using the TRIzol reagent (Invitrogen) according to manufacturer’s instructions, whereas from caryopses it was isolated by a LiCl based method. The resulting RNA was treated with RNase-free DNase I (Promega) according to the manufacturer’s protocol. Following digestion, nucleotides were removed from RNA using a G50 sepharose buffer exchange column (Amersham). Absence of g...
Monocyte-derived dendritic cells (moDCs) dramatically increase in numbers upon infection and inflammation; accordingly, we found that this also occurs during allogeneic responses. Despite their prominence, how emergent moDCs and resident conventional DCs (cDCs) divide their labor as APCs remain undefined. Hence, we compared both direct and indirect presentation by murine moDCs versus cDCs. We f...
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