To the Editor—The serologic profile of poliovirus neutralizing antibody in patients with acute leukemia prior to vaccination against poliovirus and after treatment for leukemia revealed that almost 11% of patients had protective antibody to all 3 poliovirus serotypes. After 12 months of vaccination, only 47% of patients had protective antibody to all 3 poliovirus serotypes. The antibody titer c...

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Poliovirus IRES-mediated translation requires the functions of certain canonical as well as non-canonical factors for the recruitment of ribosomes to the viral RNA. The interaction of cellular proteins PCBP2 and SRp20 in extracts from poliovirus-infected cells has been previously described, and these two proteins were shown to function synergistically in viral translation. To further define the...

In 1988, the World Health Assembly of the World Health Organization (WHO) resolved to eradicate polio worldwide. Among the three wild poliovirus (WPV) types (type 1, type 2, and type 3), WPV type 2 (WPV2) has been eliminated in the wild since 1999, and WPV type 3 (WPV3) has not been reported since 2012. In 2015, only Afghanistan and Pakistan have reported WPV transmission. On May 25, 2015, all ...

Because it appears that the last case of poliomyelitis caused by transmission of indigenous wild poliovirus occurred 2 years ago on 23 August 2992 in Peru, the chalienge for PAHO is to develop a methodology of certification that will convince the rest of the world that the eradication of poliomyelitis in the Americas has been achieved. To that end, the guidelines set forth here allow for a rigo...

Polioviruses isolated from 70 acute flaccid paralysis patients from the Democratic Republic of Congo (DRC) during 2004-2011 were characterized and found to be vaccine-derived type 2 polioviruses (VDPV2s). Partial genomic sequencing of the isolates revealed nucleotide sequence divergence of up to 3.5% in the viral protein 1 capsid region of the viral genome relative to the Sabin vaccine strain. ...

During viral infections, the host secretory pathway is crucial for both innate and acquired immune responses. For example, the export of most proinflammatory and antiviral cytokines, which recruit lymphocytes and initiate antiviral defenses, requires traffic through the host secretory pathway. To investigate potential effects of the known inhibition of cellular protein secretion during poliovir...

Seventy-eight poliovirus strains isolated from river water and sewage in Toyama Prefecture, Japan, during 1993 to 1995 were characterized by the PCR-restriction fragment length polymorphism (RFLP) method and by partially sequencing the VP3 and VP1 regions of the viral genome. Of these isolates, 36 were identified as Sabin vaccine strains, and 42 were identified as vaccine variant strains that h...

The goal of polio eradication in the Western Pacific Region is within reach. The last case of polio seen in the region occurred in Cambodia in March 1997 and the region aims to be certified polio-free by the World Health Organization (WHO) in the year 2000. The Global Commission for Certification of Eradication of Poliomyelitis has specified three criteria to be fulfilled by countries in order ...

The Sabin I poliovirus live, attenuated vaccine strain encodes for four amino acid changes (i.e., D53N, Y73H, K250E, and T362I) in the RNA-dependent RNA polymerase (RdRp). We have previously shown that the T362I substitution leads to a lower fidelity RdRp, and viruses encoding this variant are attenuated in a mouse model of poliovirus. Given these results, it was surprising that the nucleotide ...