Transmembrane lactate movements are mediated by monocarboxylate transporters (MCTs), but these proteins have never been characterized in rainbow trout. Our goals were to clone potential trout MCTs, determine tissue distribution, and quantify the effects of exhausting exercise on MCT expression. Such information could prove important to understand the mechanisms underlying the classic "lactate r...

Rainbow trout (Oncorhynchus mykiss) display longitudinal and developmental shifts in muscle relaxation rate. This study aimed to determine the role of variations in parvalbumin content in modulating muscle relaxation. Parvalbumin is a low molecular weight protein that buffers myoplasmic Ca2+ and enhances muscle relaxation. In some fish, longitudinal variations in muscle relaxation have been lin...

Endocrine effects in the aquatic environment are in the focus of scientists and media along with debates on the necessity of further steps in wastewater treatment. In the present study VTG responses were compared to evaluate upgrades at wastewater treatment plants (WWTPs). We investigated several advanced sewage treatment technologies at two WWTPs connected to the Schussen, a tributary of Lake ...

Energy intake and genetic background are major determinants of muscle fat content in most animals, including man. We combined genetic selection and dietary energy supply to study the metabolic pathways involved in genetic and nutritional control of fat deposition in the muscle of rainbow trout (Oncorhynchus mykiss). Two experimental lines of rainbow trout, selected for lean (L) or fat (F) muscl...

Expression of the Mx protein of rainbow trout was analyzed after induction by poly I:C dsRNA and infectious hematopoietic necrosis virus (IHNV). Poly I:C dsRNA-treated rainbow trout gonad (RTG-2) cells expressed Mx protein that was detectable by immunoblot analysis at 24 h post induction. Increased expression was observed at 48 h and then declined by 72 h post induction. In contrast, IHNV was n...

Proliferative kidney disease (PKD), caused by the myxozoan parasite Tetracapsuloides bryosalmonae, is well documented as a seasonal disease of rainbow trout Oncorhynchus mykiss. Water temperatures influence the course of the infection both within the fish and the invertebrate host, the recovery of fish from the disease being accelerated with decreasing water temperatures. During this study, gro...

An outbreak of listeriosis in Sweden, consisting of nine cases, was investigated by means of molecular typing of strains from patients and strains isolated from suspected foodstuffs, together with interviews of the patients. Listeria monocytogenes was isolated from six of the patients, and all isolates were of the same clonal type. This clonal type was also isolated from a "gravad" rainbow trou...

The relationship between virulence and overall within-host fitness of the fish rhabdovirus Infectious hematopoietic necrosis virus (IHNV) was empirically investigated in vivo for two virus isolates belonging to different IHNV genogroups that exhibit opposing host-specific virulence. U group isolates are more virulent in sockeye salmon and M group isolates are more virulent in rainbow trout. In ...

Cation concentration and water content were determined in the normal rainbow trout cornea, aqueous humor, lens, and vitreous humor. Anatomically the rainbow trout cornea possesses a thicker epithelium and a more prominent Bowman's membrane than those found in mammalian corneas. Descemet's membrane and the endothelium appear more primitive than their mammalian counterparts. In vitro, the cornea ...

Dichloroacetate selectively improves cardiac function and metabolism in female and male rainbow 1 trout 2 3 Pavan K. Battiprolu* and Kenneth J. Rodnick 4 5 6 Department of Biological Sciences, Idaho State University, Pocatello, ID 83209-8007 7 8 *Current address: Department of Metabolic disorders, Amgen, Inc. South San Francisco, CA 94568. 9 10 Corresponding author. e-mail: [email protected]; ph...