In experimental science, organisms are usually studied in isolation, but in the wild, they compete and cooperate in complex communities. We report a system for cross-kingdom communication by which bacteria heritably transform yeast metabolism. An ancient biological circuit blocks yeast from using other carbon sources in the presence of glucose. [GAR(+)], a protein-based epigenetic element, allo...

A method has been developed for the cloning of plasma membrane transporters by screening yeast transformed with a cDNA library for the accumulation of radiolabelled substrate. The applicability of the method is demonstrated by cloning the amino acid permease AAP1. A yeast mutant defective in proline uptake was transformed with an Arabidopsis thaliana cDNA library and plated on medium supplement...

A brewing yeast strain, NCYC 1085, unusual in that it sporulated freely and produced diploid spores, was sensitive to the degree of osmotic tension induced by the addition of 1.5 M KC1 or 2.5 M ethylene glycol to yeast extract-peptone glucose medium, but its progeny, obtained on sporulation and dissection of the resulting asci, included a number of osmotic-tolerant strains, the percentage of wh...

The Bateson-Dobzhansky-Muller (BDM) model of reproductive isolation by genetic incompatibility is a widely accepted model of speciation. Because of the exceptionally rich biological information about the budding yeast Saccharomyces cerevisiae, the identification of BDM incompatibilities in yeast would greatly deepen our understanding of the molecular genetic basis of reproductive isolation and ...

CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast directly from clinical specimens, including stool, urine, respiratory, vaginal, orop...

Yeast aldolase was purified partially by Warburg and Christian (I), who found that it was inhibited by metal-binding agents and could be reactivated by addition of Fe++, Co++, or Zn++ ions. Later, this enzyme was further purified, treated with a chelating agent, and crystallized as a mercury salt (2). In this form, the enzyme was still active when tested in the presence of cysteine and Zn++ ion...

Alcoholic fermentation is a polimicrobial process involving a large number of genera and species of yeast and bacteria. Th e major yeast genera involved in this process belongs to the Saccharomyces spp. Th e aim of this study was the isolation, identifi cation and determination of genetic diversity of indigenous Saccharomyces cerevisiae natural population taken from cv. Plavac mali from seclude...

background and objectives: acetic acid bacteria (aab) are useful in industrial production of vinegar. the present study aims at isolation and identification of acetic acid bacteria with characterization, optimization, and evaluation of their acetic acid productivity. materials and methods: samples from various fruits were screened for presence of acetic acid bacteria on glucose, yeast extract, ...

An increasing number of studies of hybridization in recent years have revealed that complete reproductive isolation between species is frequently not finalized in more or less closely related organisms. Most of these species do, however, seem to retain their phenotypical characteristics despite the implication of gene flow, highlighting the remaining gap in our knowledge of how much of an organ...

OBJECTIVE To evaluate CHROMagar Candida (CA), a new yeast differential medium, for yeast isolation in a clinical laboratory for the routine examination of high vaginal swabs. METHODS Results of high vaginal swab cultures processed in a standard manner on plates containing equal halves of Sabouraud dextrose agar (SDA) and CA were compared. Non-Candida albicans yeast isolates were further speci...