In Argentina Bovine Genital Campylobacteriosis is routinely diagnosed by direct immunofluorescence test. Generally, the hyperimmune sera used for this test are obtained from rabbits and less often from goats. In this work, a chicken egg yolk immunoglobulin (IgY) extract was conjugated and its ability to detect campylobacters with the regular conjugate prepared with rabbit sera was comparatively...

Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum....

Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labele...

Egg yolk oil is a natural source of bioactive compounds such as DHA, fat–soluble vitamins, lutein, phospholipids and cholesterol. These important compounds are also found in breast milk: DHA for infant brain development, lutein for eye health, vitamins A and E to support developing cells. Egg yolk oil naturally contains vitamin D which is required for a normal bone development. Fatty acid profi...

Yolk steroids of maternal origin have been proposed to influence genetic sex determination in birds, based on sex differences in yolk steroid concentrations of peafowl eggs incubated for 10 days. More recent reports dispute this proposal, as yolk steroids in eggs incubated for 3 days do not show such sex differences. To date, research examining this phenomenon has only analysed incubated eggs, ...

In this study, 100 day-old broiler chicks were divided into two groups, A and B containing 50 birds each. Experimental infection with E. coli was given intra yolk to group A on day-1 of experiment, while group B was kept as control. Parameters studied were examination of yolk sac, yolk sac:body weight ratio, antibody titer against Newcastle disease virus (NDV) and analysis of fractional serum p...

Thyroid hormones (THs) are essential for the correct development of nearly every structure in the body from the very early stages of development, yet the embryonic thyroid gland is not functional at these stages. To clarify the roles of the egg yolk as a source of THs, the TH content in the yolk and the expression of TH regulator genes in the yolk sac membrane were evaluated throughout the 21-d...

UV light inactivation of Mycobacterium avium subsp. paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. UV treatment proved to be less effective in killing M. avium subsp. paratuberculosis suspended in milk (0.5- to 1.0-log(10) reduction per 1,000 mJ/ml) than that s...

We have recently established a unique in vitro experimental model for mitochondrion-rich cell (MRC) research, a ;yolk-ball' incubation system, in which the yolk sac is separated from the embryonic body of Mozambique tilapia embryos and subjected to in vitro incubation. To evaluate the ion-transporting property of the yolk balls, we examined Cl- content and turnover in yolk balls incubated in fr...

abstract  introduction: this experiment was conducted to study the effects of different levels of flaxseed (0, 5, 10 and 15% of diet) and copper (in the form of cupric sulfate pentahydrate, 0 and 250 ppm of diet) on fatty acids and cholesterol in egg yolk of laying hens. methods: a total of 128 white leghorn layers aged 50 weeks were used in a factorial design and randomly assigned to 8 treatme...