In Escherichia coli the synthesis of phosphatidic acid from glycerophosphate involves first the intermediate formation of 1-acyl glycerophosphate. This reaction is catalyzed by the membrane-bound acyl-CoA(acyl-ACP):glycerophosphate acyltransferase. The 1-acylglycerophosphate is then converted to phosphatidic acid by acyl-CoA(acyl-ACP):1-acylglycerophosphate acyltransferase (Okuyama, H., and Wak...

1. Glycerol kinase (EC 2.7.1.30) is shown to catalyse a non-equilibrium reaction in rat liver; and, as it is the first enzyme in the pathway metabolizing glycerol, its properties may be pertinent to the metabolic regulation of glycerol uptake and utilization by this tissue. 2. The properties of hepatic glycerol kinase were studied by using a radiochemical technique to measure the enzyme activit...

Thermoreversible biogels can serve as effective systems for delivery of drugs through nose with increased nasal residence time. The objective of this study was to use chitosan and glycerophosphate based thermoreversible systems for delivery of doxepin to brain through intranasal administration. Formulations were prepared by admixture of suitable dilutions of chitosan and glycerophosphate with o...

Inhibition of purified AMP-deaminase from chicken breast muscle by phosphate esters and inorganic phosphate has been investigated. When tested at a concentration of 3 mM, in the presence of 0.15 M potassium chloride, the order of effectiveness as inhibitors of deaminase activity was: 2,3diphosphoglyceric acid, 3-phosphoglyceric acid, 2-phosphoglyceric acid, 2-phosphoenolpyruvate, fructose 1,6di...

Passaged primary cultures of young adult rat bone marrow cells were maintained in medium containing combinations of the supplements dexamethasone, ascorbic acid and Na-{3-glycerophosphate. The cultures were examined by both light and scanning electron microscopy (with additional energy dispersive X-ray analysis). In cultures with fully supplemented medium, an extracellular matrix formed at the ...

OBJECTIVE This in vitro study evaluated the effect of calcium glycerophosphate (CaGP) supplemented to soft drinks on bovine enamel erosion. MATERIAL AND METHODS Four pH-cycles were performed, alternating demineralization by the beverage and remineralization in artificial saliva. RESULTS Mean wear (± SD, µm) was 7.91 ± 1.13, 7.39 ± 1.01, 7.50 ± 0.91 and 5.21 ± 1.08 for Coca-Cola® without CaG...

Within the past few years considerable progress has been made in regard to the possible pathways of phospholipide synthesis in the liver. Kornberg and Pricer (1) have reported that in microsomal fractions of guinea pig liver ar-glycerophosphate is a precursor of phosphatidic acid, which is formed by an acylation of glycerophosphate by coenzyme A derivatives of fatty acid. In a similar manner, K...

A study of the fine structural localization of the acyltransferases of the monoglyceride and alpha-glycerophosphate pathways for triglyceride synthesis in the intestinal absorptive cell is reported. Glutaraldehyde-fixed tissue was found to synthesize diglyceride and triglyceride from monopalmitin and palmityl CoA, and parallel morphological studies showed the appearance of lipid droplets in the...