The adhesiveness of integrin L 2 is modulated by divalent cations. We mutated three metal ion-binding sites in the 2 I domain. The metal ion-dependent adhesion site (MIDAS) and the ligandinduced metal-binding site are required for ligand binding and sufficient for synergism between Ca2 and Mg2 . Adjacent to MIDAS (ADMIDAS) mutants are constitutively active but remain bent, with poor exposure of...

Laminins regulate diverse cellular functions through interaction with integrins. Two regions of laminins—three laminin globular domains of the a chain (LG1–3) and the carboxyl-terminal tail of the g chain (g-tail)—are required for integrin binding, but it remains unclear how the g-tail contributes to the binding. We determined the crystal structure of the integrin binding fragment of laminin-51...

Based on a large span continuous rigid frame bridge in Chongqing of China, the main pier vertical displacement and deviation, the bridge deck alignment and the expansion joint deformation are analytically researched during operation. Firstly, the monitoring content and method of the large span continuous rigid frame bridge are clearly stated. Secondly, by finite element software Midas Civil, th...

An overview of the conception and development of the MIDAS system at Arizona State University is given: a Microscope for Imaging, Diffraction and Analysis of Surfaces. John Cowley's vision in the early 1980s was ambitious and far-reaching, and it was because of him the authors came to ASU. We were centrally involved in the design and implementation of MIDAS from the mid 1980s onwards; the novel...

MIDAS is a model-driven methodology for Web Information Systems development based on the OMG’s Model Driven Architecture. It proposes to use UML to describe different views of the system. For PIM and PSM levels, MIDAS defines different aspects including content, hypertext and functionality, and, for each aspect, it also defines models and mappings between them. Content Models represent how the ...

Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for identifying physiologically relevant, low-affinity metabolite-protein interactions using unmodified proteins and complex mixtures of unmodified metabolites. In a pilot experiment u...

Inventory carrying costs represent a considerable portion of business logistics costs for manufacturing, and include the costs of interest, taxes, obsolescence, depreciation, insurance, and warehousing. Using an automated supply chain management system, these business logistics costs, particularly the inventory carrying costs and the logistics administration costs, can be reduced. The MIDAS sys...

We investigate how Global Economic Policy Uncertainty (GEPU) drives the long-run components of volatilities and correlations in crude oil and U.S. industry-level stock markets. Using the modified generalized autoregressive conditional heteroskedasticity mixed data sampling (GARCH-MIDAS) and dynamic conditional correlation mixed data sampling (DCC-MIDAS) specifications, we find that GEPU is posi...

Image segmentation, the process of separating the elements within a picture, is frequently used for obtaining information from photomicrographs. Segmentation methods should be used with reservations, since incorrect results can mislead when interpreting regions of interest (ROI). This decreases the success rate of extra procedures. Multi-Level Starlet Segmentation (MLSS) and Multi-Level Starlet...