human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as...

in this work, removal of c. i. acid red 14 was investigated by pumice stone as a low cost adsorbent. various parameters such as initial dye solution, contact time and ph were studied. removal of dye were increased by increasing of contact time and initial dye solution and deceased by increasing of ph. three isotherm models were studied. linear and non-linear regression analyses were used for de...

the feasibility of kaolinite used as a low-cost adsorbent for the removal of pb(ii), zn(ii) and cu(ii) from aqueous solutions was investigated. during the removal process, batch technique was used, and the effects of heavy metal concentration and contact time on adsorption efficiency at ph of 4.5, under a constant temperature of 20?1 ?c were studied. the experimental results were analyzed using...

We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reac...

Suppose that we have one run of n observations of a stochastic process by means of computer simulation and would like to construct a condifence interval for the steady-state mean of the process. Seeking for independent observations, so that the classical statistical methods could be applied, we can divide the n observations into k batches of length m (n= k.m) or alternatively, transform the cor...

front of the batch processor, where they wait to be processed. The batch processor has a finite capacity Q, and the processing time is independent of the number of jobs loaded into the batch processor. In certain manufacturing systems (including semiconductor wafer fabrication), a processing time window exists from the time the job exits the feeder processor till the time it enters the batch pr...

Increasing numbers of filter materials have been proposed as suitable media for P removal in on-site wastewater treatment systems. The phosphorus sorption capacity (PSC) of the material can be estimated in batch experiments and is commonly used as the criterion for material selection. However, there is no standard procedure and batch experimental parameters are arbitrarily established, thus lea...

A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only...