BACKGROUND Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level o...

The mitogen reactivity of peripheral blood and bone marrow cells from 5 patients with T-cell chronic (T-CLL) and 9 patients with T-ceII acute (T-ALL) Iymphoproliferative disease was investigated. Proliferative responsiveness to phytohemagglutinin (PHA) generally was found to be reduced or completely absent. Stimulation with PHA in the presence of the tumor promoter 1 2-0-tetradecanoyl phorbol-1...

The kinetics of ribosomal RNA transcription and processing were assessed in chronic lymphocytic leukemia (CLL) lymphocytes during the initial phases of their delayed response to phytohemagglutinin. When compared to cultures of normal lymphocytes, CLL cultures developed normal augmentations in 45S rRNA precursor transcription and cleavage after a 1 hr incubation with PHA. However, failure to con...

Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia, B-cell type, were purified into T, helper T, and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3, OKT4, and OKT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production ...

XRD and EDX analyses confirmed the presence of Ag in all coatings deposited using electrochemical method and silver. The mean thickness coating was noted to be highest in EHA/AG group (102.20 4.20 um) followed by PHA (76.40 2.20 um). Mean coating thicknesses in other groups were: PHA/AgNO3 (76.20 1.29 um); EHA (32.98 2.50 um); EHA/ Ag/2-layers (30.70 2.40 um); EHA/AgNO3 (8.60 0.60 um). The aver...

= Abstract = Predictability for positive (or negativel of the three test protocols (RPHA only; RPHA-PHA; RPHA-PHA-CORABl, which were commonly used in assessing hepatitis B virus (HBVl infection, were evaluated with the reference method of RIA for HBsAg, anti-HBc and anti-HBs in 123 adults in Seoul. RPHA-PHA-CORAB and RPHA-PHA protocols were of great value in estimating prevalence, especially wh...

PURPOSE OF REVIEW This review provides background on the formation of the Partnership for a Healthier America (PHA), that was created in conjunction with the Let's Move! initiative, and an overview of its work to date. RECENT FINDINGS To encourage industry to offer and promote healthier options, PHA partners with the private sector. Principles that guide PHA partnerships include ensuring that...

This work investigates the environmental impacts of three manufacturing processes for a promising biodegradable polymeric material: polyhydroxyalkanoate (PHA). The processes studied are microbial PHA manufacturing using glucose as the carbon substrate, microbial PHA manufacturing using cheese whey as the carbon substrate, and microbial PHA manufacturing using genetically engineered plants. A li...

Immunosuppressive effect of pregnant serum obtained in the second and third trimesters was demonstrated in the PHA-induced homologous lymphocyte stimulation system, where 3H-thymidine incorporation into DNA was measured. Such an inhibitory factor was not present in the first trimester serum. The immunosuppressive principle detected in the second trimester serum was characterized by a potent inh...

The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coli. The IgG b...