The effect of sucrose concentration on the micropropagation axillary shoots willow was investigated. following factors were examined: culture system (semisolid medium in glass jars versus liquid temporary immersion bioreactors), type explant (apical and basal sections), frequency immersion, CO2 enrichment. Shoots leaf growth significantly higher RITA® bioreactors than for all treatments. Apical...

Acca sellowiana (Berg) Burret is a fruit species native to southern Brazil and Uruguay. Micropropagation techniques have been developed in order scale-up the mass propagation of new varieties. Micropropagated plants display anatomical, morphological physiological changes, which may negativelly affect their initial development acclimatization phase. The objective this work was compare morphometr...

Plant tissue culture refers to growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic and controlled environment. Micropropagation allows rapid production of high quality, disease-free and uniform planting material. The micropropagation of high quality planting materials of ornamentals, and forest and fruit trees has created new opportun...

background: stevia rebaudiana bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. objective: the present study was conducted to optimize a protocol for rapid micropropagation of stevia rebaudiana by shoot tip explants and to invest...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

one of the most effective ways to propagate lilium is by using tissue culture techniques, preferably through bulblet production. in addition to the surviving percentage of lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing lilium micropropagation. different concentratio...