Clinical samples obtained over a period of 8 months (n = 2,624) were processed in parallel with the BACTEC 460TB system, with the MGIT 960 system, and in Löwenstein-Jensen (LJ) medium, resulting in the recovery of 127 mycobacteria. Recovery rates in combinations of the BACTEC 460TB or MGIT 960 system with LJ were, respectively, 94.7 and 94.7% for Mycobacterium tuberculosis complex (n = 57) and ...

The use of the COBAS AMPLICOR System (Roche Molecular Diagnostics, Basel, Switzerland), the only automated system for PCR testing, was evaluated for a rapid identification of mycobacteria with positive BACTEC 12B cultures. Two hundred ninety-six specimens with a growth index of >/=30 were analyzed for the presence of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium int...

Objective: In the study, 419 Mycobacterium tuberculosis complex strains (MTC), which were isolated from samples sent to Regional Tuberculosis Laboratory in our city between 2015 and 2019 with suspected Erzurum surrounding cities investigated. Methods: Our research is a retrospective cross-sectional study clinical Public Health suspicion of within five-year period January December first evaluate...

A single bottle blood culture system that detects the gas produced by micro-organisms from substrates in a specially formulated medium was compared with the Bactec radiometric system. A total of 725 blood samples collected from patients with suspected bacteraemia yielded 75 clinically important isolates. Fifty five (73%) of these cultures were detected by both methods, 16 (21%) by only the Bact...

برای دستیابی به مزیت رقابتی در شرایط عدم‌­اطمینان که آن تغییر ضروری است، یکی از چالش‌های بزرگ سازمان‌ها کاهش ریسک طریق ایجاد زنجیره‌های تأمین تاب‌آور است. تاب‌آوری زنجیره ‌تأمین توانایی مقابله با اختلال اشاره دارد یک رویداد غیرقابل‌پیش‌بینی بوده و دارای منابع داخلی خارجی مختلفی ازجمله بلایای طبیعی ریسک‌های عملیاتی پژوهش حاضر شبیه‌سازی توسط نرم‌افزار ارنا زنجیره‌ سنگ ساختمانی «کارخانه سنگبری آسم...

OBJECTIVES To describe wild-type distributions of the MIC of fluoroquinolones for Mycobacterium tuberculosis in relation to current critical concentrations used for drug susceptibility testing and pharmacokinetic/pharmacodynamic (PK/PD) data. METHODS A 96-stick replicator on Middlebrook 7H10 medium was used to define the MICs of ciprofloxacin, ofloxacin, moxifloxacin and levofloxacin for 90 c...

Results and costs of the first six months experience with BACTEC NR-730 were compared with a series of blood cultures performed by the conventional method previously used. The newer technology detected the growth of 14.1% of significant isolates on the day of receipt of the specimens. The previous method lacked blind subcultures on the day of receipt and therefore detected growth only after ove...

Introduction of PCR to directly detect Mycobacterium tuberculosis in clinical specimens has shown promise; however, interfering substances in clinical material have contributed to lowered assay sensitivities. We evaluated the ability of a PCR assay to detect M. tuberculosis in BACTEC 12B broth cultures. Clinical specimens were processed and inoculated into BACTEC 12B vials. Evaluation was appro...

An American Type Culture Collection reference strain and eight clinical strains of Mycobacterium tuberculosis, all of which were susceptible to isoniazid (INH) (mean MIC, 0.06 mg/liter) and negative for the Ser315Thr katG mutation, were left in their BACTEC 12B vials (for use with the BACTEC 460-TB method) containing 0.1 mg of INH per liter for periods of up to 28 days after the completion of t...