We present a theoretical study of the low frequency vibrational modes of the M13 bacteriophage using a fully atomistic model. Using ideas from electronic structure theory, the few lowest vibrational modes of the M13 bacteriophage are determined using classical harmonic analysis. The relative Raman intensity is estimated for each of the mechanical modes using a bond polarizability model. Compari...

Mutations that cause an increased level of expression of the histidine transport operon were isolated and characterized genetically. Five independently isolated promoter-up mutations were transferred to an M13 hybrid phage that carries the histidine transport operon, and their nucleotide sequences were determined. For all five mutations, the change was the same as the one previously determined ...

Self-assembly of nanostructured materials has been proven a powerful technique in material design and synthesis. By phage display screening, M13 phage was found to strongly bind sulfur particles. Fourier transform infrared and X-ray photoelectron spectroscopy measurements indicated that the strong sulfur-binding ability of M13 phage derives from newly generated S-O and C-S bonds. Using this pha...

The Effect of hydroxy isothiocyanates on a bacterial virus and M13 DNA was examined. Hydroxy-substituted phenyl and phenyl alkyl isothiocyanates, especially 2-(3,4-dihydroxyphenyl)ethyl isothiocyanate(IT-Dop) synthesized from dopamine, showed antiviral activity on psiK. In transfection experiments with M13 mp DNA species, IT-Dop inhibited the single-stranded (SS) molecule more effectively than ...

as a template for PCR. Sequencing of the plasmid DNA can now be performed using two PCR primers flanking the MCS of the vector (we used M13 universal forward primer and M13 universal reverse primer; Figure 1). If the unique primer is set 100–150 bp from the end of the known sequence, sufficient overlapping sequence is generated to align the sequences unambiguously. This method was used in our l...

The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon...

4 Richard Marley and Richard A. Baines 5 Faculty of Life Sciences, University of Manchester, M13 9PT, UK. 6 7 8 Text pages: 37 9 Figures: 8 10 Tables: 1 11 Supplementary videos: 2 12 13 Running Head: Drosophila epilepsy model 14 15 Address for correspondence: 16 Richard Baines 17 1.016 Hill Building 18 Faculty of Life Sciences, 19 University of Manchester, 20 Oxford Road, 21 Manchester, 22 M13 ...

A practical method to assemble rodlike tobacco mosaic virus and bateriophage M13 with polymers was developed, which afforded a 3D core-shell composite with morphological control.

In this paper, we present a new approach to subpixel infrared (IR) emitter detection in VIIRS mid-wave (MWIR) bands M12–M13 at night, based on the presence of tightly clustered background diagonal full granule scattergrams M12 versus M13 radiances. This is found universally night-time data collected worldwide. The feature absent during day due solar reflectance. existence attributed close spaci...