Dipeptidyl peptidase IV (DPPIV), also known as CD26, is a 110-kDa cell surface glycoprotein expressed in various tissues. DPPIV reportedly plays a direct role in the progression of several human malignancies. DPPIV specific inhibitors are employed as antidiabetics and could potentially be repurposed to enhance anti-tumor immunotherapies. In the present study, we investigated the correlation bet...

BACKGROUND. The heterodimeric transcription factor HIF-1 (hypoxia-inducible factor 1), consisting of a critically regulated HIF-1a subunit and a constitutively expressed HIF-1b subunit, is a master regulator of genes involved in adaptation and survival under low-oxygen conditions. Increased levels of HIF-1 activity are associated with increased tumor aggressiveness, therapeutic resistance, and ...

BACKGROUND MicroRNAs (miRNAs) are a recently discovered class of posttranscriptional regulators of gene expression with critical functions in the angiogenesis and cardiovascular diseases; however, the details of miRNAs regulating mechanism of angiogenesis of ischemic cardiac microvascular endothelial cells (CMECs) are not yet reported. METHODS AND RESULTS This study analyzes the changes of th...

MicroRNA (miR) are small noncoding RNA molecules that regulate gene expression and play important roles during kidney development, homeostasis, and disease. Novel contributions to thefieldofcelldeath, cell cycle, andmiRregulation in the kidney have come from the Laboratory of Dong, and in the present issue of JASN, a study from this laboratory clarifies a role of miR-687 as a key regulator and ...

Regulations between NF-κB and HIF-1 have not been adequately addressed in previous research. Here, we report that hypoxia increased NF-κB in hepatocellular carcinoma cells. The HIF-1 protein level was rapidly induced by protein stabilization (by 2 hours) and then moderately decreased, whereas mRNA levels were reciprocally increased. We also found that NF-κB p50 and p65 (RelA), but not c-Rel, bo...

Mevcut çalışmamız, sporda genetik etmenlerin damar mekanizması üzerindeki etkisini incelemek amacıyla yapılmıştır. Damarlar, sportif performansın etkili ve verimli bir şeklide sürdürülmesinde anahtar role sahip olabilir. Anjiyogenez, mevcut damarlardan birtakım fizyolojik süreçler yoluyla yeni oluşumudur. bugüne kadar VEGFA, HIF-1A, ACE NOS3 genleri ile ilgili NCBI veri tabanına kayıtlı; PubMed...

Hypoxia strongly up-regulates tissue factor and promotes plasma clotting by glioblastoma multiforme, but transcriptional mechanisms remain undefined. Here, we investigated the potential roles of early growth response gene-1 (Egr-1), Sp1, nuclear factor-KB (NF-KB), activator protein-1 (AP-1), and hypoxia-inducible factor-1 (HIF-1) in the hypoxic regulation of tissue factor by glioblastoma multif...

Top1 inhibition by camptothecin (CPT) perturbs RNA polymerase II (Pol II) density at promoters and along transcribed genes suggesting an involvement of Top1 in Pol II pausing. Here, we demonstrate that Top1 inhibition favors Pol II escape from a promoter-proximal pausing site of the human HIF-1a gene in living cells. Interestingly, alternative splicing at exon 11 was markedly altered in nascent...